Chapter title |
Propagation, Purification, and Characterization of Bacteriophages for Phage Therapy.
|
---|---|
Chapter number | 22 |
Book title |
Bacteriophages
|
Published in |
Methods in molecular biology, January 2024
|
DOI | 10.1007/978-1-0716-3549-0_22 |
Pubmed ID | |
Book ISBNs |
978-1-07-163548-3, 978-1-07-163549-0
|
Authors |
Kosznik-Kwaśnicka, Katarzyna, Topka, Gracja, Mantej, Jagoda, Grabowski, Łukasz, Necel, Agnieszka, Węgrzyn, Grzegorz, Węgrzyn, Alicja, Katarzyna Kosznik-Kwaśnicka, Gracja Topka, Jagoda Mantej, Łukasz Grabowski, Agnieszka Necel, Grzegorz Węgrzyn, Alicja Węgrzyn |
Abstract |
Phage therapy is an alternative approach to combat bacterial infections. In this approach, bacteriophages are used as antimicrobial agents due to their properties to infect specific bacterial cells, to propagate inside their hosts, and to lyse host cell to release progeny phages. However, to introduce bacteriophages to clinical or veterinary practice, it is necessary to construct a large library of precisely characterized phages. Therefore, in this chapter, methods for propagation, purification, and microbiological characterization of bacteriophages are presented in the light of their potential use in phage therapy. Isolation of newly discovered bacteriophages from different habitats is also described as it is a preliminary assessment of their efficacy in combating bacterial biofilms and in the treatment of bacterial infections in a simple insect model-Galleria mellonella. |
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