Chapter title |
Expression and Functional Studies of Leaf, Floral, and Fruit Developmental Genes in Non-model Species.
|
---|---|
Chapter number | 19 |
Book title |
Flower Development
|
Published in |
Methods in molecular biology, January 2023
|
DOI | 10.1007/978-1-0716-3299-4_19 |
Pubmed ID | |
Book ISBNs |
978-1-07-163298-7, 978-1-07-163299-4
|
Authors |
Pabón-Mora, Natalia, Suárez-Baron, Harold, Madrigal, Yesenia, Alzate, Juan F, González, Favio, Alzate, Juan F. |
Abstract |
Researchers working on evolutionary developmental plant biology are inclined to choose non-model taxa to address how specific features have been acquired during ontogeny and fixed during phylogeny. In this chapter we describe methods to extract RNA, to assemble de-novo transcriptomes, to isolate orthologous genes within gene families, and to evaluate expression and function of target genes. We have successfully optimized these protocols for non-model plant species including ferns, gymnosperms, and a large assortment of angiosperms. In the latter, we have ranged a large number of families including Aristolochiaceae, Apodanthaceae, Chloranthaceae, Orchidaceae, Papaveraceae, Rubiaceae, Solanaceae, and Tropaeolaceae. |
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