| Chapter title |
Immunodetection of Human LINE-1 Expression in Cultured Cells and Human Tissues.
|
|---|---|
| Chapter number | 17 |
| Book title |
Transposons and Retrotransposons
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3372-3_17 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3370-9, 978-1-4939-3372-3
|
| Authors |
Reema Sharma, Nemanja Rodić, Kathleen H. Burns M.D., Ph.D., Martin S. Taylor, Kathleen H. Burns |
| Editors |
Jose L. Garcia-Pérez |
| Abstract |
Long interspersed element-1 (LINE-1) is the only active protein-coding retrotransposon in humans. It is not expressed in somatic tissue but is aberrantly expressed in a wide variety of human cancers. ORF1p protein is the most robust indicator of LINE-1 expression; the protein accumulates in large quantities in cellular cytoplasm. Recently, monoclonal antibodies have allowed more complete characterizations of ORF1p expression and indicated potential for developing ORF1p as a clinical biomarker. Here, we describe a mouse monoclonal antibody specific for human LINE-1 ORF1p and its application in immunofluorescence and immunohistochemistry of both cells and human tissues. We also describe detection of tagged LINE-1 ORF2p via immunofluorescence. These general methods may be readily adapted to use with many other proteins and antibodies. |
Mendeley readers
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|---|---|---|
| Unknown | 22 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
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| Student > Master | 4 | 18% |
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| Researcher | 2 | 9% |
| Student > Doctoral Student | 1 | 5% |
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|---|---|---|
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| Unspecified | 1 | 5% |
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| Other | 1 | 5% |
| Unknown | 4 | 18% |