Chapter title |
Label-Based and Label-Free Strategies for Protein Quantitation.
|
---|---|
Chapter number | 4 |
Book title |
Proteome Bioinformatics
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-6740-7_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6738-4, 978-1-4939-6740-7
|
Authors |
Sushma Anand, Monisha Samuel, Ching-Seng Ang, Shivakumar Keerthikumar, Suresh Mathivanan, Anand, Sushma, Samuel, Monisha, Ang, Ching-Seng, Keerthikumar, Shivakumar, Mathivanan, Suresh |
Editors |
Shivakumar Keerthikumar, Suresh Mathivanan |
Abstract |
The precise quantification of changes between various physiological states in a biological system is highly complex in nature. Over the past few years, in combination with classical methods, mass spectrometry based approaches have become an indispensable tool in deciphering exact abundance of proteins in composite mixtures. The technique is now well established and employs both label-based and label-free quantitation strategies. Label-based quantitation methods utilize stable isotope labels which are incorporated within the peptides, introducing an expectable mass difference within the two or more experimental conditions. In contrast, label-free proteomics quantitates both relative and absolute protein quantity by utilizing signal intensity and spectral counting of peptides. This chapter focuses on the commonly used quantitative mass spectrometry methods for high-throughput proteomic analysis. |
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Student > Ph. D. Student | 15 | 14% |
Researcher | 12 | 11% |
Student > Master | 12 | 11% |
Student > Postgraduate | 7 | 6% |
Other | 14 | 13% |
Unknown | 32 | 29% |
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Chemistry | 8 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 5% |
Medicine and Dentistry | 5 | 5% |
Other | 15 | 14% |
Unknown | 35 | 32% |