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Plant Iron Homeostasis

Overview of attention for book
Cover of 'Plant Iron Homeostasis'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 High-Throughput Plant Gene Expression Analysis by 384-Format Reverse Transcription-Quantitative PCR for Investigating Plant Iron Homeostasis.
  3. Altmetric Badge
    Chapter 2 The Use of Spectral Imaging to Follow the Iron and pH-Dependent Accumulation of Fluorescent Coumarins.
  4. Altmetric Badge
    Chapter 3 Assay of Fe(III) Chelate Reductase Activity in Arabidopsis thaliana Root.
  5. Altmetric Badge
    Chapter 4 An Adapted Protocol for Quantitative Rhizosphere Acidification Assay.
  6. Altmetric Badge
    Chapter 5 Techniques to Study Common Root Responses to Beneficial Microbes and Iron Deficiency.
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    Chapter 6 Imaging and Quantifying the Endocytosis of IRON-REGULATED TRANSPORTER1 from Arabidopsis.
  8. Altmetric Badge
    Chapter 7 Label-Free Quantitative Proteomics in Plant.
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    Chapter 8 Chromatin Immunoprecipitation (ChIP) to Study the Transcriptional Regulatory Network that Controls Iron Homeostasis in Arabidopsis thaliana.
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    Chapter 9 Comprehensive Survey of ChIP-Seq Datasets to Identify Candidate Iron Homeostasis Genes Regulated by Chromatin Modifications.
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    Chapter 10 Arabidopsis Micro-grafting to Study the Systemic Signaling of Nutrient Status
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    Chapter 11 Advances in Iron Retrograde Signaling Mechanisms and Uptake Regulation in Photosynthetic Organisms.
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    Chapter 12 Functional Analysis of Chloroplast Iron Uptake and Homeostasis.
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    Chapter 13 Perls/DAB Staining to Examine Iron Distribution in Arabidopsis Embryos
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    Chapter 14 Visualizing Metal Distribution in Plants Using Synchrotron X-Ray Fluorescence Microscopy Techniques
  16. Altmetric Badge
    Chapter 15 A Simple Semi-hydroponic System for Studying Iron Homeostasis in Maize.
  17. Altmetric Badge
    Chapter 16 Optimizing Fe Nutrition for Algal Growth.
Attention for Chapter 5: Techniques to Study Common Root Responses to Beneficial Microbes and Iron Deficiency.
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (79th percentile)
  • High Attention Score compared to outputs of the same age and source (93rd percentile)

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Chapter title
Techniques to Study Common Root Responses to Beneficial Microbes and Iron Deficiency.
Chapter number 5
Book title
Plant Iron Homeostasis
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-3183-6_5
Pubmed ID
37166592
Book ISBNs
978-1-07-163182-9, 978-1-07-163183-6
Authors

Hsu, Shu-Hua, Stassen, Max J J, Pieterse, Corné M J, Stringlis, Ioannis A, Stassen, Max J. J., Pieterse, Corné M. J., Stringlis, Ioannis A.

Abstract

Iron (Fe) plays a central role in the vital processes of a plant. The Fe status of a plant influences growth and immunity, but it also dictates interactions of roots with soil microbiota through the production of Fe mobilizing, antimicrobial fluorescent phenolic compounds called coumarins. To adapt to low Fe availability in the soil, plants deploy an efficient Fe deficiency response. Interestingly, this Fe deficiency response is hijacked by root-colonizing microbes in the root microbiome to establish a mutually beneficial relationship. In this chapter, we describe how we cultivate plants and microbes to study the interaction between plants, beneficial rhizobacteria, and the plant's Fe deficiency response. We describe (a) how we study activity and localization of these responses by assessing gene-specific promoter activities using GUS assays, (b) how we visualize root-secreted coumarins in response to Fe deficiency and colonization by beneficial rhizobacteria, and (c) how we prepare our samples for metabolite extraction and reverse-transcriptase quantitative PCR to analyze the expression of marker genes.

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X Demographics

X Demographics

The data shown below were collected from the profiles of 11 X users who shared this research output. Click here to find out more about how the information was compiled.
As of 1 July 2024, you may notice a temporary increase in the numbers of X profiles with Unknown location. Click here to learn more.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 1 50%
Unknown 1 50%
Readers by discipline Count As %
Agricultural and Biological Sciences 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 8. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 30 May 2023.
All research outputs
#4,984,739
of 26,004,690 outputs
Outputs from Methods in molecular biology
#1,341
of 14,508 outputs
Outputs of similar age
#100,935
of 482,853 outputs
Outputs of similar age from Methods in molecular biology
#43
of 715 outputs
Altmetric has tracked 26,004,690 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 14,508 research outputs from this source. They receive a mean Attention Score of 3.5. This one has done particularly well, scoring higher than 90% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 482,853 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 79% of its contemporaries.
We're also able to compare this research output to 715 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.

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