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Enzyme Engineering

Overview of attention for book
Cover of 'Enzyme Engineering'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability In Vivo
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    Chapter 2 Determining Enzyme Kinetics via Isothermal Titration Calorimetry
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    Chapter 3 GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria.
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    Chapter 4 Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA-Binding and Cleavage Properties
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    Chapter 5 TAL Effector Nuclease (TALEN) Engineering.
  7. Altmetric Badge
    Chapter 6 In Vitro Evolution of Enzymes
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    Chapter 7 Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In Vitro and In Vivo
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    Chapter 8 Reconstructing Evolutionary Adaptive Paths for Protein Engineering
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    Chapter 9 Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria
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    Chapter 10 FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants
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    Chapter 11 Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes
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    Chapter 12 Gene Synthesis by Assembly of Deoxyuridine-Containing Oligonucleotides
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    Chapter 13 Protein Engineering: Single or Multiple Site-Directed Mutagenesis
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    Chapter 14 Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination.
  16. Altmetric Badge
    Chapter 15 Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments
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    Chapter 16 Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis
  18. Altmetric Badge
    Chapter 17 Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter
  19. Altmetric Badge
    Chapter 18 In Vitro Directed Evolution of Enzymes Expressed by E. coli in Microtiter Plates
Attention for Chapter 14: Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination.
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Chapter title
Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination.
Chapter number 14
Book title
Enzyme Engineering
Published in
Methods in molecular biology, February 2013
DOI 10.1007/978-1-62703-293-3_14
Pubmed ID
Book ISBNs
978-1-62703-292-6, 978-1-62703-293-3
Authors

Ostrov N, Wingler LM, Cornish VW, Nili Ostrov, Laura M. Wingler, Virginia W. Cornish, Ostrov, Nili, Wingler, Laura M., Cornish, Virginia W.

Abstract

While mutagenesis of single genes is now common practice in molecular biology, engineering multiple target genes still requires complex cloning techniques and thus is limited to expert laboratories. Here, we describe "Reiterative Recombination," a user-friendly DNA assembly technique in Saccharomyces cerevisiae for the integration of an indefinite number of DNA fragments sequentially into the yeast genome. The high efficiency of chromosomal integration can further be utilized for the assembly of large combinatorial libraries for metabolic engineering.

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X Demographics

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 20 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 5%
Unknown 19 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 20%
Researcher 4 20%
Other 3 15%
Professor > Associate Professor 2 10%
Professor 2 10%
Other 3 15%
Unknown 2 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 30%
Biochemistry, Genetics and Molecular Biology 4 20%
Engineering 3 15%
Chemistry 2 10%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Other 2 10%
Unknown 2 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 May 2013.
All research outputs
#18,338,946
of 22,710,079 outputs
Outputs from Methods in molecular biology
#7,850
of 13,078 outputs
Outputs of similar age
#146,790
of 192,957 outputs
Outputs of similar age from Methods in molecular biology
#20
of 39 outputs
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So far Altmetric has tracked 13,078 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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