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Nanopore Sequencing

Overview of attention for book
Cover of 'Nanopore Sequencing'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 The Current State of Nanopore Sequencing
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    Chapter 2 Hybrid Genome Assembly of Short and Long Reads in Galaxy
  4. Altmetric Badge
    Chapter 3 Microbial Genome Sequencing and Assembly Using Nanopore Sequencers
  5. Altmetric Badge
    Chapter 4 De Novo Genome Assembly of Japanese Black Cattle as Model of an Economically Relevant Animal
  6. Altmetric Badge
    Chapter 5 How to Sequence and Assemble Plant Genomes
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    Chapter 6 Detection of DNA Modification Using Nanopore Sequencers
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    Chapter 7 Ultralow-Input Genome Library Preparation for Nanopore Sequencing with Droplet MDA
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    Chapter 8 The Method of Eliminating the Wolbachia Endosymbiont Genomes from Insect Samples Prior to a Long-Read Sequencing
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    Chapter 9 A Nanopore Sequencing Course for Graduate School Curriculum
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    Chapter 10 A Guide to Sequencing for Long Repetitive Regions
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    Chapter 11 Analysis of Tandem Repeat Expansions Using Long DNA Reads.
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    Chapter 12 Finding Rearrangements in Nanopore DNA Reads with LAST and dnarrange.
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    Chapter 13 Long-Read Whole-Genome Sequencing Using a Nanopore Sequencer and Detection of Structural Variants in Cancer Genomes.
  15. Altmetric Badge
    Chapter 14 Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens.
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    Chapter 15 Nanopore Sequencing Data Analysis of 16S rRNA Genes Using the GenomeSync-GSTK System.
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    Chapter 16 Genomic Epidemiological Analysis of Antimicrobial-Resistant Bacteria with Nanopore Sequencing.
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    Chapter 17 Rapid and Comprehensive Identification of Nontuberculous Mycobacteria.
  19. Altmetric Badge
    Chapter 18 Long-Read Single-Cell Sequencing Using scCOLOR-seq.
  20. Altmetric Badge
    Chapter 19 Unfolding the Bacterial Transcriptome Landscape Using Oxford Nanopore Technology Direct RNA Sequencing.
  21. Altmetric Badge
    Chapter 20 Nanopore Direct RNA Sequencing of Monosome- and Polysome-Bound RNA.
  22. Altmetric Badge
    Chapter 21 RNA Modification Detection Using Nanopore Direct RNA Sequencing and nanoDoc2.
Attention for Chapter 14: Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens.
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  • Average Attention Score compared to outputs of the same age
  • Above-average Attention Score compared to outputs of the same age and source (59th percentile)

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Chapter title
Full-Length 16S rRNA Gene Analysis Using Long-Read Nanopore Sequencing for Rapid Identification of Bacteria from Clinical Specimens.
Chapter number 14
Book title
Nanopore Sequencing
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-2996-3_14
Pubmed ID
Book ISBNs
978-1-07-162995-6, 978-1-07-162996-3
Authors

Matsuo, Yoshiyuki

Abstract

Amplicon sequencing of the 16S ribosomal RNA (rRNA) gene is a practical and reliable measure for taxonomic profiling of bacterial communities. This chapter describes the detailed workflow for full-length 16S rRNA gene amplicon analysis using nanopore sequencing and bioinformatics pipelines to analyze nanopore sequencing data for taxonomic assignment. This approach offers a higher taxonomic resolution for bacterial identification from clinical specimens with a markedly reduced timeframe and improved versatility.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 5 100%

Demographic breakdown

Readers by professional status Count As %
Lecturer > Senior Lecturer 1 20%
Student > Ph. D. Student 1 20%
Researcher 1 20%
Unknown 2 40%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 20%
Agricultural and Biological Sciences 1 20%
Unknown 3 60%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 February 2023.
All research outputs
#15,181,211
of 23,351,247 outputs
Outputs from Methods in molecular biology
#4,839
of 13,337 outputs
Outputs of similar age
#209,032
of 426,097 outputs
Outputs of similar age from Methods in molecular biology
#127
of 391 outputs
Altmetric has tracked 23,351,247 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,337 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 426,097 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 391 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.