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Poly(ADP-Ribose) Polymerase

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Cover of 'Poly(ADP-Ribose) Polymerase'

Table of Contents

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    Book Overview
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    Chapter 1 Fluorescence-Based Analyses of Poly(ADP-Ribose) Length by Gel Electrophoresis, High-Performance Liquid Chromatography, and Capillary Electrophoresis
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    Chapter 2 Detecting and Quantifying pADPr In Vivo
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    Chapter 3 Quantitative Analysis of Nuclear Poly(ADP-Ribose) Dynamics in Response to Laser-Induced DNA Damage
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    Chapter 4 Analyzing PARP1 Activity: Small Molecule Reactants and Attached Chains of Poly (ADP-Ribose)
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    Chapter 5 Detecting Poly (ADP-Ribose) In Vitro and in Cells Using PAR Trackers
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    Chapter 6 An Enzyme-Linked Immunosorbent Assay to Quantify Poly (ADP-Ribose) Level In Vivo
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    Chapter 7 Subcellular Quantitation of ADP-Ribosylation by High-Content Microscopy
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    Chapter 8 A Simple Method to Study ADP-Ribosylation Reversal: From Function to Drug Discovery
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    Chapter 9 Immunoprecipitation Using Mono-ADP-Ribosylation-Specific Antibodies
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    Chapter 10 A Clickable NAD+ Analog-Based Assay of Poly(ADP-Ribosyl)ated Proteins
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    Chapter 11 Functional Analysis of Histone ADP-Ribosylation In Vitro and in Cells
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    Chapter 12 Studying the Immunomodulatory Functions of PARP1 and PARP2 in Mouse Models of Cancer
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    Chapter 13 Methods for Investigating Transient Receptor Potential Melastatin-2 (TRPM2): A Cation Channel Activated by ADP-Ribose and Involved in Cell Death
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    Chapter 14 Methods to Assess the Role of PARPs in Regulating Mitochondrial Oxidative Function
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    Chapter 15 Characterizing ADP-Ribosylation Sites Using Af1521 Enrichment Coupled to ETD-Based Mass Spectrometry
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    Chapter 16 Cytological Approaches to Visualize Intracellular Dynamics of RNA-Binding Proteins at Active Genes in Drosophila
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    Chapter 17 Chromatin Immunoprecipitation Approach to Determine How PARP1 Domains Affect Binding Pattern to Chromatin
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    Chapter 18 Approach to Measuring the Effect of PARP1 on RNA Polymerase II Elongation Rates
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    Chapter 19 Examining the Effect of PARP-1 Inhibitors on Transcriptional Activity of Androgen Receptor in Prostate Cancer Cells
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    Chapter 20 Using Drosophila Genetics to Identify Factors that Affect PARP1 Activity In Vivo
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    Chapter 21 Generating PARP Knockout D. melanogaster with CRISPR/Cas9 System
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    Chapter 22 TaqMan Multiplex qPCR Method to Genotype PARG Knockout Mice
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    Chapter 23 Cell-Based Screening for New PARP Inhibitors Utilizing PARG-Mutated Mouse Embryonic Stem Cells
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    Chapter 24 Quantification of PARP7 Protein Levels and PARP7 Inhibitor Target Engagement in Cells Using a Split Nanoluciferase System
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    Chapter 25 Purification of Recombinant Human PARG and Activity Assays
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    Chapter 26 Purification of Recombinant Human PARP-3
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Title
Poly(ADP-Ribose) Polymerase
Published by
Springer US, December 2022
DOI 10.1007/978-1-0716-2891-1
ISBNs
978-1-07-162890-4, 978-1-07-162891-1
Editors

Tulin, Alexei V.

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