The Ubiquitin Code

Chapter 1 Thioester and Oxyester Linkages in the Ubiquitin System

Chapter 2 Getting to the Root of Branched Ubiquitin Chains: A Review of Current Methods and Functions

Chapter 3 Chemical Synthesis of Non-hydrolyzable Ubiquitin(-Like) Hybrid Chains

Chapter 4 Total Linear Chemical Synthesis of LC3A and LC3B

Chapter 5 A Microbead-Based Flow Cytometry Assay to Assess the Activity of Ubiquitin and Ubiquitin-Like Conjugating Enzymes

Chapter 6 Monitoring HECT Ubiquitination Activity In Vitro

Chapter 7 RNAi-Based Screening for the Identification of Specific Substrate-Deubiquitinase Pairs

Chapter 8 Strategies for Monitoring “Ubiquitin C-Terminal Hydrolase 1” (Yuh1) Activity

Chapter 9 Isolation and Mass Spectrometry Identification of K48 and K63 Ubiquitin Proteome Using Chain-Specific Nanobodies

Chapter 10 A Mass Spectrometry-Based Strategy for Mapping Modification Sites for the Ubiquitin-Like Modifier NEDD8

Chapter 11 Isolation of the Hepatic Ubiquitome/NEDDylome by Streptavidin Pull-Down Assay in the Biotinylated Ubiquitin (bioUb)/Biotinylated NEDD8 (bioNEDD8) Transgenic Mice

Chapter 12 The Lambda Display Technology: A Useful Tool for the Identification of Ubiquitin-and Ubiquitin-Like-Binding Domains

Chapter 13 SUMO-ID: A Strategy for the Identification of SUMO-Dependent Proximal Interactors

Chapter 14 Analysis of ATG8 Family Members Using LC3-Interacting Regions (LIR)-Based Molecular Traps

Chapter 16 In-Plate and In-Gel Assays for the Assessment of Proteasome Activity in  Caenorhabditis elegans

Chapter 17 Isolation of Proteasome-Trapped Peptides (PTPs) for Degradome Analysis