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Plant Proteostasis

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Cover of 'Plant Proteostasis'

Table of Contents

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    Book Overview
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    Chapter 1 Observing Real-Time Ubiquitination in High Throughput with Fluorescence Polarization
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    Chapter 2 Identification and Characterization of Physiological Pairing of E2 Ubiquitin-Conjugating Enzymes and E3 Ubiquitin Ligases
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    Chapter 3 Immunoprecipitation of Cullin–Ring Ligases (CRLs) in Arabidopsis thaliana Seedlings
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    Chapter 4 An In vitro Assay to Recapitulate Hormone-Triggered and SCF-Mediated Protein Ubiquitylation
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    Chapter 5 Analysis of Proteasome-Associated Ubiquitin Ligase Activity
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    Chapter 6 Measuring the DUB Activity of Arabidopsis Deubiquitylating Enzymes
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    Chapter 7 SUMO Conjugation and SUMO Chain Formation by Plant Enzymes
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    Chapter 8 Kinetic Analysis of Plant SUMO Conjugation Machinery
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    Chapter 9 Expression, Purification, and Enzymatic Analysis of Plant SUMO Proteases
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    Chapter 10 Monitoring Autophagic Flux in the Model Single-Celled Microalga Chlamydomonas reinhardtii
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    Chapter 11 Detection of Autophagy in Plants by Fluorescence Microscopy
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    Chapter 12 Characterization of ATG8-Family Interactors by Isothermal Titration Calorimetry
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    Chapter 13 Protocols for Studying Protein Stability in an Arabidopsis Protoplast Transient Expression System
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    Chapter 14 Relative Protein Lifetime Measurement in Plants Using Tandem Fluorescent Protein Timers
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    Chapter 15 Detection and Quantification of Protein Aggregates in Plants
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    Chapter 16 Using Intrinsic Fluorescence to Measure Protein Stability Upon Thermal and Chemical Denaturation
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    Chapter 17 Purification and Detection of Ubiquitinated Plant Proteins Using Tandem Ubiquitin Binding Entities
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    Chapter 18 Titanium Oxide-Based Phosphopeptide Enrichment from Arabidopsis Seedlings
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    Chapter 19 Chloroplast Envelope Membrane Subfractionation from Arabidopsis and Pea
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    Chapter 20 Chromatin Enrichment for Proteomics in Plants (ChEP-P)
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    Chapter 21 Proximity-Dependent In Vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha
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    Chapter 22 Tandem Mass Tag-Based Phosphoproteomics in Plants
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    Chapter 23 Analysis of Peptide Hormone Maturation and Processing Specificity Using Isotope-Labeled Peptides
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    Chapter 24 Improved Identification of Protease Cleavage Sites by In-gel Reductive Dimethylation
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    Chapter 25 A Pipeline to Monitor Proteasome Homeostasis in Plants
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    Chapter 26 Bioinformatic Tools for Exploring the SUMO Gene Network: An Update
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    Chapter 27 Coexpression Network Construction and Visualization from Transcriptomes Underlying ER Stress Responses
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    Chapter 28 Approaches for the Identification of Intrinsically Disordered Protein Domains
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Title
Plant Proteostasis
Published by
Springer US, November 2022
DOI 10.1007/978-1-0716-2784-6
ISBNs
978-1-07-162783-9, 978-1-07-162784-6
Editors

Lois, L. Maria, Trujillo, Marco

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