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SARS-CoV-2

Overview of attention for book
Cover of 'SARS-CoV-2'

Table of Contents

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    Book Overview
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    Chapter 1 Evolution and Epidemiology of SARS-CoV-2 Virus
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    Chapter 2 Molecular Epidemiology of SARS-CoV-2 by Sequencing
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    Chapter 3 Advanced Genetic Methodologies in Tracking Evolution and Spread of SARS-CoV-2
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    Chapter 4 Antigen-Based Point of Care Testing (POCT) for Diagnosing SARS-CoV-2: Assessing Performance
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    Chapter 5 Diagnostic Method for COVID-19 Using Sugar Chain-Immobilized Nanoparticles and Saliva Specimens
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    Chapter 6 Detection and Quantification of SARS-CoV-2 by Real-Time RT-PCR Assay
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    Chapter 7 Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans
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    Chapter 8 Propagation and Quantification of SARS-CoV-2
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    Chapter 9 Quantification of Infectious SARS-CoV-2 by the 50% Tissue Culture Infectious Dose Endpoint Dilution Assay
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    Chapter 10 One-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification
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    Chapter 11 Profiling SARS-CoV-2 Infection by High-Throughput Shotgun Proteomics.
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    Chapter 12 Detection of SARS-CoV-2 by Mass Spectrometry
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    Chapter 13 Bioinformatics-Based Approaches to Study Virus-Host Interactions During SARS-CoV-2 Infection
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    Chapter 14 Human Nasal Epithelial Cells (hNECs ) Generated by Air-Liquid Interface (ALI) Culture as a Model System for Studying the Pathogenesis of SARS-CoV-2
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    Chapter 15 Animal Models of COVID-19: Nonhuman Primates
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    Chapter 16 Animal Models of COVID-19: Transgenic Mouse Model
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    Chapter 17 Immunohistochemical Detection of SARS-CoV-2 Antigens by Single and Multiple Immunohistochemistry
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    Chapter 18 Measuring Neutralizing Antibodies to SARS-CoV-2 Using Lentiviral Spike-Pseudoviruses
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    Chapter 19 Antiviral Strategies Against SARS-CoV-2: A Systems Biology Approach
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    Chapter 20 Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test
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    Chapter 21 Pseudovirus-Based Assays for the Measurement of Antibody-Mediated Neutralization of SARS-CoV-2
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    Chapter 22 Cytopathic Effect (CPE )-Based Drug Screening Assay for SARS-CoV-2
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    Chapter 23 Strengthening Biorisk Management in Research Laboratories with Security-Sensitive Biological Agents Like SARS-CoV-2
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    Chapter 24 Biorisk Management for SARS-CoV-2 Research in a Biosafety Level-3 Core Facility
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    Chapter 25 Methods of SARS-CoV-2 Inactivation
Attention for Chapter 7: Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans
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Chapter title
Immunofluorescent Antibody Techniques in the Diagnosis of SARS-CoV-2 Infection in Humans
Chapter number 7
Book title
SARS-CoV-2
Published in
Methods in molecular biology, January 2022
DOI 10.1007/978-1-0716-2111-0_7
Pubmed ID
Book ISBNs
978-1-07-162110-3, 978-1-07-162111-0
Authors

Hueston, Linda

Abstract

Immunofluorescence (IF) is an important technique used in the diagnosis of many infectious diseases. In virology, it has proven to be particularly suited to detecting antibody directed against newly emerging viruses able to be cultivated in cell culture. It permits visualization of antibody and allows for antibody class to be determined which is critical to understanding the timing of infection. The procedure used to determine IgG, IgA, and IgM antibody directed against SARS-CoV-2 in humans is described in this chapter. These methods were developed for routine diagnosis of SARS-CoV-2 infection in Australia at the start of the global pandemic in 2020.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 2 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 2 100%

Demographic breakdown

Readers by professional status Count As %
Lecturer > Senior Lecturer 1 50%
Unknown 1 50%
Readers by discipline Count As %
Nursing and Health Professions 1 50%
Unknown 1 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 May 2022.
All research outputs
#18,146,485
of 23,312,088 outputs
Outputs from Methods in molecular biology
#7,400
of 13,318 outputs
Outputs of similar age
#344,914
of 507,944 outputs
Outputs of similar age from Methods in molecular biology
#291
of 594 outputs
Altmetric has tracked 23,312,088 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,318 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 507,944 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 594 others from the same source and published within six weeks on either side of this one. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.