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Phase-Separated Biomolecular Condensates

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Cover of 'Phase-Separated Biomolecular Condensates'

Table of Contents

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    Book Overview
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    Chapter 1 Calculating Binodals and Interfacial Tension of Phase-Separated Condensates from Molecular Simulations with Finite-Size Corrections
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    Chapter 2 Field-Theoretic Simulation Method to Study the Liquid–Liquid Phase Separation of Polymers
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    Chapter 3 Numerical Techniques for Applications of Analytical Theories to Sequence-Dependent Phase Separations of Intrinsically Disordered Proteins
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    Chapter 4 An Introduction to the Stickers-and-Spacers Framework as Applied to Biomolecular Condensates
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    Chapter 5 Multiscale Modeling of Protein-RNA Condensation in and Out of Equilibrium
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    Chapter 6 Fluorescence Lifetime Imaging Microscopy of Biomolecular Condensates
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    Chapter 7 Single-Molecule Fluorescence Methods to Study Protein-RNA Interactions Underlying Biomolecular Condensates
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    Chapter 8 Fluorescence Correlation Spectroscopy and Phase Separation
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    Chapter 9 Measurement of Protein and Nucleic Acid Diffusion Coefficients Within Biomolecular Condensates Using In-Droplet Fluorescence Correlation Spectroscopy
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    Chapter 10 Single-Molecule Imaging of the Phase Separation-Modulated DNA Compaction to Study Transcriptional Repression
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    Chapter 11 Phase Separation-Based Biochemical Assays for Biomolecular Interactions
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    Chapter 12 Determining Thermodynamic and Material Properties of Biomolecular Condensates by Confocal Microscopy and Optical Tweezers
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    Chapter 13 A High-Throughput Method to Profile Protein Liquid-Liquid Phase Separation
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    Chapter 14 Phase Separation of Rubisco by the Folded SSUL Domains of CcmM in Beta-Carboxysome Biogenesis
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    Chapter 15 Cryo-Electron Tomography of Reconstituted Biomolecular Condensates
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    Chapter 16 Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells
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    Chapter 17 Synthetic Organelles for Multiple mRNA Selective Genetic Code Expansions in Eukaryotes
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    Chapter 18 Single-Molecule Tracking of RNA Polymerase In and Out of Condensates in Live Bacterial Cells
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    Chapter 19 An Optogenetic Toolkit for the Control of Phase Separation in Living Cells
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    Chapter 20 Assessing the Phase Separation Propensity of Proteins in Living Cells Through Optodroplet Formation
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    Chapter 21 Mass Balance Imaging: A Phase Portrait Analysis for Characterizing Growth Kinetics of Biomolecular Condensates
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    Chapter 22 Characterizing Properties of Biomolecular Condensates Below the Diffraction Limit In Vivo
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Title
Phase-Separated Biomolecular Condensates
Published by
Springer US, October 2022
DOI 10.1007/978-1-0716-2663-4
ISBNs
978-1-07-162662-7, 978-1-07-162663-4
Editors

Zhou, Huan-Xiang, Spille, Jan-Hendrik, Banerjee, Priya R.

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