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Nucleic Acid Aptamers

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Cover of 'Nucleic Acid Aptamers'

Table of Contents

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    Book Overview
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    Chapter 1 Automated ssDNA SELEX Using Histidine-Tagged Target Proteins
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    Chapter 2 Discovery of Aptamers Against Cell Surface Markers Using Ligand-Guided Selection
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    Chapter 3 Implementation of Emulsion PCR for Amplification of Click-Modified DNA During SELEX
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    Chapter 4 Preparation of Chemically Modified DNA Library for SELEX via Incorporation of CLB-dUTP in Primer Extension Reaction
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    Chapter 5 RNA Capture-SELEX on Streptavidin Magnetic Beads
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    Chapter 6 The Bioinformatics of Aptamers: HT-SELEX Analysis with AptaSUITE
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    Chapter 7 Improvement of Aptamers by High-Throughput Sequencing of Doped-SELEX
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    Chapter 8 Analysis of Aptamer-Small Molecule Binding Interactions Using Isothermal Titration Calorimetry
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    Chapter 9 Determination of Aptamer Structure Using Circular Dichroism Spectroscopy
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    Chapter 10 Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis
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    Chapter 11 Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance
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    Chapter 12 Probing of Fluorogenic RNA Aptamers via Supramolecular Förster Resonance Energy Transfer with a Universal Fluorescent Nucleobase Analog
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    Chapter 13 Slow-Off-Rate-Modified Aptamer Labeling for Fluorescence Microscopy and DNA-PAINT
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    Chapter 14 Aptamer–Field-Effect Transistors for Small-Molecule Sensing in Complex Environments
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    Chapter 15 Aptasensor for Impedimetric Detection of Lysozyme
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    Chapter 16 Biophysical Analysis of Small Molecule Binding to Viral RNA Structures
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    Chapter 17 Designing and Expressing Circular RNA Aptamers to Regulate Mammalian Cell Biology
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    Chapter 18 Enzyme-Linked Oligonucleotide Assay (ELONA)
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    Chapter 19 High-Throughput Development and Optimization of RNA-Based Fluorogenic Biosensors of Small Molecules Using Droplet-Based Microfluidics
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    Chapter 20 Robotic APTamer-Enabled Electrochemical Reader (RAPTER) System for Automated Aptamer-Mediated Electrochemical Analysis
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Title
Nucleic Acid Aptamers
Published by
Springer US, September 2022
DOI 10.1007/978-1-0716-2695-5
ISBNs
978-1-07-162694-8, 978-1-07-162695-5
Editors

Mayer, Günter, Menger, Marcus M.

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