Endocannabinoid Signaling

Table of Contents

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   Book Overview
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   Chapter 1 Need for Methods to Investigate Endocannabinoid Signaling
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   Chapter 2 Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
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   Chapter 3 Measuring the Content of Endocannabinoid-Like Compounds in Biological Fluids: A Critical Overview of Sample Preparation Methodologies
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   Chapter 4 LC–MS/MS Analysis of AEA and 2-AG
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   Chapter 5 Analysis of Omega-3 Fatty Acid-Derived N-Acylethanolamines in Biological Matrices
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   Chapter 6 Endocannabinoid-Binding Receptors as Drug Targets
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   Chapter 7 Assay of CB1 Receptor Binding
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   Chapter 8 Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 9 Fluorescence-Based Assay for TRPV1 Channels
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    Chapter 10 DNA–Protein-Interaction (DPI)-ELISA Assay for PPAR-γ Receptor Binding
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    Chapter 11 Scintillation Proximity Assay (SPA)-Based Radioligand Binding for PPARα, PPARγ, and PPARδ Receptors
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    Chapter 12 Homogeneous Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Cofactor Recruitment Assay for PPARα and PPARγ
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    Chapter 13 Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 14 Assay of GTPγS Binding in Autoradiography
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    Chapter 15 Cellular Assay to Study β-Arrestin Recruitment by the Cannabinoid Receptors 1 and 2
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    Chapter 16 Endocannabinoid Metabolism and Transport as Drug Targets
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    Chapter 17 Assay of NAT Activity
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    Chapter 18 Radiometric Assay of NAPE-PLD Activity
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    Chapter 19 Fluorescence-Based NAPE-PLD Activity Assay
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    Chapter 20 Radiometric Assay of FAAH Activity
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    Chapter 21 Fluorimetric Assay of FAAH Activity
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    Chapter 22 Assay of NAAA Activity
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    Chapter 23 Assay of DAGLα/β Activity
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    Chapter 24 Assay of Monoacylglycerol Lipase Activity
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    Chapter 25 Radiometric Assay of ABHD2 Activity
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    Chapter 26 Oxygenation of Anandamide by Lipoxygenases
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    Chapter 27 Assay of Endocannabinoid Oxidation by Cytochrome P450
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    Chapter 28 Assay of Endocannabinoid Uptake
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    Chapter 29 Assessing Gene Expression of the Endocannabinoid System Components by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction
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    Chapter 30 Bioinformatics of the Endocannabinoid System: Study of DNA Methylation at Rat Cnr1 Gene Promoter
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    Chapter 31 DNA Methylation Analysis of Cnr1 Gene Promoter
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    Chapter 32 Western Blotting of the Endocannabinoid System
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    Chapter 33 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
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    Chapter 34 Assessing CB1 Expression in the Brain by Immunohistochemical Methods: Light, Confocal, and Electron Microscopy
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    Chapter 35 Measuring Endocannabinoid System Interaction with Biomembranes
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    Chapter 36 STORM Super-Resolution Imaging of CB 1 Receptors in Tissue Preparations
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    Chapter 37 Visualization of Endocannabinoids in the Cell
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    Chapter 38 Electrophysiology of Endocannabinoid Signaling
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    Chapter 39 Machine Learning and Computational Chemistry for the Endocannabinoid System
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    Chapter 40 Virtual Reverse Screening Approach to Target Type 2 Cannabinoid Receptor