Lipopolysaccharide Transport

Chapter 1 Use of Mutagenesis and Functional Screens to Characterize Essential Genes Involved in Lipopolysaccharide Transport

Chapter 2 Generation of Stable and Unmarked Conditional Mutants in Pseudomonas aeruginosa

Chapter 4 Membrane Fractionation by Isopycnic Sucrose Density Gradient Centrifugation for Qualitative Analysis of LPS in Escherichia coli

Chapter 5 Exploring the Topology of Cytoplasmic Membrane Proteins Involved in Lipopolysaccharide Biosynthesis by in Silico and Biochemical Analyses

Chapter 6 Use of Site-Directed Spin Labeling EPR Spectroscopy to Study Protein–LPS Interactions

Chapter 7 A Photo-Crosslinking Approach to Monitoring the Assembly of an LptD Intermediate with LptE in a Living Cell

Chapter 8 Mass Spectrometry Analysis of Dynamics and Interactions of the LPS Translocon LptDE

Chapter 9 Affinity Purification and Coimmunoprecipitation of Transenvelope Protein Complexes in Gram-Negative Bacteria

Chapter 10 A Bacterial Two-Hybrid System for In Vivo Assays of Protein-Protein Interactions and Drug Discovery

Chapter 11 Detecting Lipoproteins Sneaking Out of the Lipopolysaccharide Leaflet

Chapter 12 Dissecting Lipopolysaccharide Composition and Structure by GC-MS and MALDI Spectrometry

Chapter 13 Probing the Functional Interaction Interface of Lipopolysaccharide and Antimicrobial Peptides: A Solution-State NMR Perspective

Chapter 14 Cryo-EM Analysis of the Lipopolysaccharide Flippase MsbA

Chapter 15 Protein Crystallization of Two Recombinant Lpt Proteins

Chapter 16 Metabolic Incorporation of Azido-Sugars into LPS to Enable Live-Cell Fluorescence Imaging

Chapter 17 Use of Atomic Force Microscopy to Characterize LPS Perturbations