If you would like to discover more about how to de-orphanise T-cells using whole genome CRISPR-cas9, @MatLegut, @TcellResearch and I wrote a method chapter for the book on 'T-Cell Repertoire Characterization', published in Methods in Molecular Biology. ht
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PROTOCOL "Ligand Identification for Orphan MHC-Agnostic T-Cell Receptors by Whole Genome #CRISPR–Cas9 Screening" Crowther MD, Legut M, Sewell AK. In: Huang, H., Davis, M.M. (eds) T-Cell Repertoire Characterization. Methods Mol Biol 2022-09-11. https://t.
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Ligand Identification for Orphan MHC-Agnostic T-Cell Receptors by Whole Genome CRISPR-Cas9 Screening by Michael D Crowther https://t.co/sJInKkK52U