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Membrane Trafficking

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Cover of 'Membrane Trafficking'

Table of Contents

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    Book Overview
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    Chapter 1 Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System
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    Chapter 2 Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo
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    Chapter 3 Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH)
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    Chapter 4 Spatial and Temporal Control of Protein Secretion with Light
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    Chapter 5 Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing
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    Chapter 6 Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting.
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    Chapter 7 Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy
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    Chapter 8 Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique
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    Chapter 9 Live-Cell Superresolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons
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    Chapter 10 Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse
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    Chapter 11 In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion
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    Chapter 12 Imaging Single-Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)
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    Chapter 13 Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy
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    Chapter 14 Endocytosis Assays Using Cleavable Fluorescent Dyes
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    Chapter 15 Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2
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    Chapter 16 Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches
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    Chapter 17 Measuring Plasma Membrane Recycling Using Microscopic and Biochemical Approaches
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    Chapter 18 Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins
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    Chapter 19 Measurement of Lysosome Positioning by Shell Analysis and Line Scan
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    Chapter 20 Optogenetic Control of Membrane Trafficking Using Light-Activated Reversible Inhibition by Assembly Trap of Intracellular Membranes (IM-LARIAT)
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    Chapter 21 Generating Custom Pooled CRISPR Libraries for Genetic Dissection of Biological Pathways
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    Chapter 22 Studying Unconventional Secretion of Misfolded Proteins in Cultured Cells and Primary Neurons
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    Chapter 23 Single-Molecule Optical Tweezers Study of Protein–Membrane Interactions
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    Chapter 24 Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes
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    Chapter 25 Quantitative Measurement of Extracellular Vesicle Content Delivery Within Acceptor Cells
Attention for Chapter 16: Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches
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