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Mitochondria

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Cover of 'Mitochondria'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Fast Determination of Mitochondrial Metabolism and Respiratory Complex Activity in Permeabilized and Intact Cells.
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    Chapter 2 Unbiased Millivolts Assay of Mitochondrial Membrane Potential in Intact Cells
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    Chapter 3 Rotenone Decreases Ischemia-Induced Injury by Inhibiting Mitochondrial Permeability Transition: A Study in Brain
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    Chapter 4 Assessment of Mitochondrial Complex II and III Activity in Brain Sections: A Histoenzymological Technique
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    Chapter 5 Measurement of Mitochondrial (Dys)Function in Cellular Systems Using Electron Paramagnetic Resonance (EPR): Oxygen Consumption Rate and Superoxide Production
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    Chapter 6 Mitochondrial Calcium Handling in Isolated Mitochondria from a Guinea Pig Heart
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    Chapter 7 Characterizing the Electron Transport Chain: Structural Approach
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    Chapter 8 Characterizing the Electron Transport Chain: Functional Approach Using Extracellular Flux Analyzer on Mouse Tissue Samples
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    Chapter 9 Simultaneous Acquisition of Mitochondrial Calcium Retention Capacity and Swelling to Measure Permeability Transition Sensitivity
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    Chapter 10 Measuring Mitochondrial Function: From Organelle to Organism.
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    Chapter 11 Mitochondrial Toxicity of Organic Arsenicals
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    Chapter 12 Measurements of Mitochondrial Respiration in Intact Cells, Permeabilized Cells, and Isolated Tissue Mitochondria Using the Seahorse XF Analyzer
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    Chapter 13 Monitoring Mitochondrial Morphology and Respiration in Doxorubicin-Induced Cardiomyopathy
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    Chapter 14 Creation of Yeast Models for Evaluating the Pathogenicity of Mutations in the Human Mitochondrial Gene MT-ATP6 and Discovering Therapeutic Molecules
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    Chapter 15 In Vivo Analysis of Mitochondrial Protein Synthesis in Saccharomyces cerevisiae Mitochondrial tRNA Mutants
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    Chapter 16 Visualizing Mitochondrial Importability of a Protein Using the Yeast Bi-Genomic Mitochondrial-Split-GFP Strain and an Ordinary Fluorescence Microscope
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    Chapter 17 Analysis of Mitochondrial Performance in Lymphocytes Using Fluorescent Lifetime Imaging Microscopy
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    Chapter 18 Monitoring Mitochondrial Perturbations During Infection
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    Chapter 19 Measuring the Mitochondrial Ubiquinone (Q) Pool Redox State in Isolated Respiring Mitochondria
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    Chapter 20 Experimental Setup for Investigation of Acute Mitochondrial Oxygen Sensing in Primary Cells
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    Chapter 21 Assessing the Redox Status of Mitochondria Through the NADH/FAD2+ Ratio in Intact Cells
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    Chapter 22 Monitoring Mitochondrial Membrane Potential in Live Cells Using Time-Lapse Fluorescence Imaging
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    Chapter 23 Investigating Mitochondrial Ca2+ Dynamics in Isolated Mitochondria and Intact Cells: Application of Fluorescent Dyes and Genetic Reporters
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    Chapter 24 A Plate Reader-Based Measurement of the Cellular ROS Production Using Dihydroethidium and MitoSOX
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    Chapter 25 Analysis of Organization and Activity of Mitochondrial Respiratory Chain Complexes in Primary Fibroblasts Using Blue Native PAGE
  27. Altmetric Badge
    Chapter 26 Multiplexing Seahorse XFe24 and ImageXpress® Nano Platforms for Comprehensive Evaluation of Mitochondrial Bioenergetic Profile and Neuronal Morphology
  28. Altmetric Badge
    Chapter 27 Computational Modeling of Mitochondria to Understand the Dynamics of Oxidative Stress
  29. Altmetric Badge
    Chapter 28 Correction to: Mitochondria
Attention for Chapter 10: Measuring Mitochondrial Function: From Organelle to Organism.
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Chapter title
Measuring Mitochondrial Function: From Organelle to Organism.
Chapter number 10
Book title
Mitochondria
Published in
Methods in molecular biology, January 2022
DOI 10.1007/978-1-0716-2309-1_10
Pubmed ID
Book ISBNs
978-1-07-162308-4, 978-1-07-162309-1
Authors

Lewis, Matthew T, Levitsky, Yan, Bazil, Jason N, Wiseman, Robert W, Lewis, Matthew T., Bazil, Jason N., Wiseman, Robert W.

Abstract

Mitochondrial energy production is crucial for normal daily activities and maintenance of life. Herein, the logic and execution of two main classes of measurements are outlined to delineate mitochondrial function: ATP production and oxygen consumption. Aerobic ATP production is quantified by phosphorus magnetic resonance spectroscopy (31PMRS) in vivo in both human subjects and animal models using the same protocols and maintaining the same primary assumptions. Mitochondrial oxygen consumption is quantified by oxygen polarography and applied in isolated mitochondria, cultured cells, and permeabilized fibers derived from human or animal tissue biopsies. Traditionally, mitochondrial functional measures focus on maximal oxidative capacity-a flux rate that is rarely, if ever, observed outside of experimental conditions. Perhaps more physiologically relevant, both measurement classes herein focus on one principal design paradigm; submaximal mitochondrial fluxes generated by graded levels of ADP to map the function for ADP sensitivity. We propose this function defines the bioenergetic role that mitochondria fill within the myoplasm to sense and match ATP demands. Any deficit in this vital role for ATP homeostasis leads to symptoms often seen in cardiovascular and cardiopulmonary diseases, diabetes, and metabolic syndrome.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 43%
Student > Doctoral Student 2 29%
Researcher 1 14%
Student > Postgraduate 1 14%
Readers by discipline Count As %
Medicine and Dentistry 2 29%
Immunology and Microbiology 1 14%
Computer Science 1 14%
Sports and Recreations 1 14%
Neuroscience 1 14%
Other 0 0%
Unknown 1 14%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 July 2022.
All research outputs
#20,246,428
of 22,774,233 outputs
Outputs from Methods in molecular biology
#9,866
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Outputs of similar age
#407,919
of 497,865 outputs
Outputs of similar age from Methods in molecular biology
#403
of 594 outputs
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