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Heterologous Expression of Membrane Proteins

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Cover of 'Heterologous Expression of Membrane Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Solubilization and Purification of α5β1 Integrin from Rat Liver for Reconstitution into Nanodiscs
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    Chapter 2 Membrane Protein Production in Escherichia coli : Protocols and Rules
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    Chapter 3 Functional Overexpression of Membrane Proteins in E. coli: The Good, the Bad, and the Ugly
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    Chapter 4 Heterologous Expression of Membrane Proteins in E. coli
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    Chapter 5 Overexpression of the ABC Transporter BmrA Within Intracellular Caveolae in Escherichia coli
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    Chapter 6 Production of Membrane Proteins in Pseudomonas stutzeri
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    Chapter 7 Overproduction of Membrane-Associated, and Integrated, Proteins Using Saccharomyces cerevisiae
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    Chapter 8 Purification of Membrane Proteins Overexpressed in Saccharomyces cerevisiae
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    Chapter 9 Production and Purification of a GFP-Tagged ABC Transporter CaCdr1p
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    Chapter 10 Membrane Protein Production in the Yeast Yeasts P. pastoris
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    Chapter 11 Production and Preparation of Isotopically Labeled Human Membrane Proteins in Pichia pastoris for Fast-MAS-NMR Analyses Fast-MAS-NMR analyses
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    Chapter 12 Membrane Protein Production in Insect Cells
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    Chapter 13 Thromboxane A2 G Protein-Coupled Receptor Production and Crystallization for Structure Studies
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    Chapter 14 Production of Human ABC Transporters and Oligosaccharyltransferase Complexes for Structural Studies
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    Chapter 15 Heterologous Expression and Purification of GPCRs
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    Chapter 16 Expression and Purification of the Human Thyroid-Stimulating Hormone Receptor
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    Chapter 17 Optimization of Recombinant GPCR Proteins for Biophysical and Structural Studies Using Virus-like Particles
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    Chapter 18 Peptide Tags and Domains for Expression and Detection of Mammalian Membrane Proteins at the Cell Surface
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    Chapter 19 Tailoring Purification and Analysis of Membrane Proteins with Modular Detergents
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    Chapter 20 Detergent Alternatives: Membrane Protein Purification Using Synthetic Nanodisc Polymers
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    Chapter 21 Detergent-Free Membrane Protein Purification Using SMA Polymer
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    Chapter 22 Cell-Free Expression of GPCRs into Nanomembranes for Functional and Structural Studies
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    Chapter 23 Cell-Free Expression of Proton-Coupled Folate Transporter in the Presence of Nanodiscs Nanodiscs
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    Chapter 24 Microscale Thermophoresis to Evaluate the Functionality of Heterologously Overexpressed Membrane Proteins in Membrane Preparations
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