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Induced Pluripotent Stem (iPS) Cells

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Cover of 'Induced Pluripotent Stem (iPS) Cells'

Table of Contents

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    Book Overview
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    Chapter 299 The Characteristics of Human iPS Cells and siRNA Transfection Under Hypoxia
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    Chapter 300 Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells
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    Chapter 301 Pluripotent Stem Cell Differentiation Toward Functional Basal Stratified Epithelial Cells
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    Chapter 302 mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions
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    Chapter 303 Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells
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    Chapter 304 Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity
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    Chapter 318 Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells
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    Chapter 323 Highly Efficient Differentiation of Human Pluripotent Stem Cells into Pancreatic Progenitors Co-expressing PDX1 and NKX6.1
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    Chapter 327 Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells
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    Chapter 328 Isolation and Culture of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells
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    Chapter 334 Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells
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    Chapter 335 Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes
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    Chapter 336 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells
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    Chapter 337 CRISPR/Cas9–Mediated Gene Knockout and Knockin Human iPSCs
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    Chapter 348 Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System
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    Chapter 349 Derivation of Clinical-Grade Induced Pluripotent Stem Cell Lines from Erythroid Progenitor Cells in Xenofree Conditions
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    Chapter 350 Generation of Murine Induced Pluripotent Stem Cells through Transposon-Mediated Reprogramming
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    Chapter 351 The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts
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    Chapter 352 CRISPR/Cas9 Ribonucleoprotein Complex-Mediated Efficient B2M Knockout in Human Induced Pluripotent Stem Cells (iPSCs)
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    Chapter 353 Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes
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    Chapter 354 Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells
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    Chapter 355 Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage
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    Chapter 356 A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors
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    Chapter 357 3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells
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    Chapter 358 Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation
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    Chapter 359 Human Pluripotent Stem Cell Differentiation to Microglia
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    Chapter 360 A Simple Method for Generating, Clearing, and Imaging Pre-vascularized 3D Adipospheres Derived from Human iPS Cells
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    Chapter 361 A Method for In Vitro Fabrication of Hybrid Bone/Cartilage Tissue Using Mouse Induced Pluripotent Stem Cells
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    Chapter 362 CRISPR/Cas9-Mediated Genome Editing to Generate Clonal iPSC Lines
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    Chapter 363 Differentiation of Human Induced Pluripotent Stem Cells (hiPSC) into Endothelial-Type Cells and Establishment of an In Vitro Blood-Brain Barrier Model
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    Chapter 364 Non-modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells
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    Chapter 368 CRISPR/Cas9-Mediated Introduction of Specific Heterozygous Mutations in Human Induced Pluripotent Stem Cells
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    Chapter 369 Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology
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    Chapter 370 The Differentiation of Human Induced Pluripotent Stem Cells into Podocytes In Vitro
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    Chapter 372 Generation of Human Induced Pluripotent Stem Cells Using Endothelial Progenitor Cells Derived from Umbilical Cord Blood and Adult Peripheral Blood
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    Chapter 373 Efficient Induction of Primate iPS Cells Using a Combination of RNA Transfection and Chemical Compounds
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    Chapter 380 Generation and Cultivation of Transgene-Free Macaque and Baboon iPSCs Under Chemically Defined Conditions
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    Chapter 381 Generation of Marmoset Monkey iPSCs with Self-Replicating VEE-mRNAs in Feeder-Free Conditions
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    Chapter 382 An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms.
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    Chapter 386 Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming
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    Chapter 387 Methods for Isolation and Reprogramming of Various Somatic Cell Sources into iPSCs
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    Chapter 388 Differentiation of Human Induced Pluripotent Stem Cells into Definitive Endoderm Using Simple Dialysis Culture Device
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    Chapter 391 Efficient High-Density hiPSCs Expansion in Simple Dialysis Device
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    Chapter 392 Derivation of Three-Dimensional Human Induced Pluripotent Stem Cell-Derived Vocal Fold Mucosa for Clinical and Pharmacological Applications
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    Chapter 393 Development of a Blood–Brain Barrier Permeability Assay Using Human Induced Pluripotent Stem Cell Derived Brain Endothelial Cells
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    Chapter 394 Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
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    Chapter 395 Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
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    Chapter 408 Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells
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    Chapter 414 Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures.
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    Chapter 428 Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces
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    Chapter 429 Human Induced Pluripotent Stem Cell-Derived Microglia (hiPSC-Microglia).
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    Chapter 430 Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells
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    Chapter 443 Revised “hPSC-Sac Method” for Simple and Efficient Differentiation of Human Pluripotent Stem Cells to Hematopoietic Progenitor Cells
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    Chapter 445 Generation of Induced Pluripotent Stem Cells from Human Bone Marrow–Derived Mesenchymal Stem Cells
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    Chapter 446 Porcine iPSC Generation: Testing Different Protocols to a Successful Application
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    Chapter 458 Correction to: Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
Attention for Chapter 382: An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms.
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Chapter title
An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms.
Chapter number 382
Book title
Induced Pluripotent Stem (iPS) Cells
Published in
Methods in molecular biology, May 2021
DOI 10.1007/7651_2021_382
Pubmed ID
33982275
Book ISBNs
978-1-07-162118-9, 978-1-07-162119-6
Authors

Izadifar, Mohammad, Berecz, Tünde, Apáti, Ágota, Nagy, Andras

Abstract

Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) hold great promise for cardiovascular disease modeling, drug screening and personalized medicine. A crucial requirement to establish an hPSC-CM-based disease model is the availability of a reliable differentiation protocol and a functional assessment of phenotypic properties of CMs in a disease context. Characterization of relative changes in contractile behavior of CMs can provide insight not only about drug effects but into the pathogenesis of cardiovascular diseases. Image-based optical-flow analysis, which applies a speckle tracking algorithm to videomicroscopy of hPSC-CMs, is a noninvasive method to quantitatively assess the dynamics of mechanical contraction of the CMs. This method offers an efficient characterization of contractile cycles. It quantifies contraction velocity field, beat rate, contractile strain and contraction-relaxation strain rate profile, which are important phenotypic characteristics of CMs.

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The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 33%
Student > Master 1 33%
Unknown 1 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 33%
Nursing and Health Professions 1 33%
Unknown 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 April 2022.
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#20,931,913
of 23,563,389 outputs
Outputs from Methods in molecular biology
#10,186
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#366,990
of 442,242 outputs
Outputs of similar age from Methods in molecular biology
#266
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