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Cancer Cell Biology

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Cover of 'Cancer Cell Biology'

Table of Contents

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    Book Overview
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    Chapter 1 Overview of the Maintenance of Authentic Cancer Cell Lines
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    Chapter 2 Culturing Suspension Cancer Cell Lines
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    Chapter 3 Culture of Adherent Cancer Cell Lines
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    Chapter 4 Breast Cancer Xenograft Murine Models
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    Chapter 5 Processing and Cryopreservation of Blood, Cancer Tissues, and Cancer Cells for Viable Biobanking
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    Chapter 6 Adipocyte–Breast Cancer Cell Co-Culture in Transwells
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    Chapter 7 Three-Dimensional Co-Culture Method for Studying Interactions Between Adipocytes, Extracellular Matrix, and Cancer Cells
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    Chapter 8 Modeling Collective Invasion and Single-Cell Mesenchymal Invasion in Three-Dimensional Matrigel–Collagen I Cultures
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    Chapter 9 CellTrace™ Violet Flow Cytometric Assay to Assess Cell Proliferation
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    Chapter 10 Enzyme-Linked Immunosorbent Assay (ELISA)
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    Chapter 11 Flow Cytometry Analysis to Detect Lapatinib-Induced Modulation of Constitutive and IFN-γ-Induced HLA Class I Expression in HER2-Positive Breast Cancer Cells
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    Chapter 12 An End-to-End Workflow for Interrogating Tumor-Infiltrating Myeloid Cells Using Mass Cytometry
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    Chapter 13 Manual Immunofluorescence of Formalin-Fixed Paraffin-Embedded Human Tumor Tissues
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    Chapter 14 Western Blot Analysis of Lapatinib-Mediated Inhibition of the Epidermal Growth Factor Receptor 2 (HER2) Pathway in Breast Cancer Cells
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    Chapter 15 Evaluating Cell Membrane Localization and Intracellular Transport of Proteins by Biotinylation
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    Chapter 16 Multiplexed Quantitative Proteomic Profiling of Cancer Cells and Tissues Using Isobaric Labeling-Based Tags.
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    Chapter 17 The Use of Seahorse XF Assays to Interrogate Real-Time Energy Metabolism in Cancer Cell Lines
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    Chapter 18 Using CRISPR-Cas9 to Dissect Cancer Mutations in Cell Lines
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    Chapter 19 Single-Cell RNA Sequencing Analysis Using Fluidigm C1 Platform for Characterization of Heterogeneous Transcriptomes
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    Chapter 20 Sequences to Differences in Gene Expression: Analysis of RNA-Seq Data
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    Chapter 21 Reverse Transcription-Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR) for Gene Expression Analyses
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    Chapter 22 Peptide-Affinity Isolation of Extracellular Vesicles and Cell-Free DNA From Human Plasma
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    Chapter 23 Characterizing Extracellular Vesicles Using Nanoparticle-Tracking Analysis
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