Chapter 1 Designing Overlap Extension PCR Primers for Protein Mutagenesis: A Programmatic Approach

Chapter 2 Recombination of Single Beneficial Substitutions Obtained from Protein Engineering by Computer-Assisted Recombination (CompassR)

Chapter 3 Nondegenerate Saturation Mutagenesis: Library Construction and Analysis via MAX and ProxiMAX Randomization

Chapter 4 Antha-Guided Automation of Darwin Assembly for the Construction of Bespoke Gene Libraries

Chapter 5 SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution

Chapter 6 Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis

Chapter 7 GeneORator: An Efficient Method for the Systematic Mutagenesis of Entire Genes

Chapter 9 Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs

Chapter 10 Application of Restriction Free (RF) Cloning in Circular Permutation

Chapter 11 Site-Directed Mutagenesis Method Mediated by Cas9

Chapter 12 Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity

Chapter 13 A Screening Method for P450 BM3 Mutant Libraries Using Multiplexed Capillary Electrophoresis for Detection of Enzymatically Converted Compounds.

Chapter 14 Directed Evolution of Glycosyltransferases by a Single-Cell Ultrahigh-Throughput FACS-Based Screening Method

Chapter 15 Learning Strategies in Protein Directed Evolution