Chapter title |
Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor.
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Chapter number | 398 |
Book title |
Bioreactors in Stem Cell Biology
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Published in |
Methods in molecular biology, May 2021
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DOI | 10.1007/7651_2021_398 |
Pubmed ID | |
Book ISBNs |
978-1-07-162017-5, 978-1-07-162018-2
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Authors |
Pennarossa, Georgia, Ghiringhelli, Matteo, Gandolfi, Fulvio, Brevini, Tiziana A L, Brevini, Tiziana A. L. |
Abstract |
Long-segment airway stenosis as well as their neoplastic transformation is life-threatening and still currently represent unsolved clinical problems. Indeed, despite several attempts, definitive surgical procedures are not presently available, and a suitable tracheal reconstruction or replacement remains an urgent clinical need. A possible innovative strategic solution to restore upper airway function may be represented by the creation of a bioprosthetic trachea, obtained through the combination of tissue engineering and regenerative medicine.Here we describe a two-step protocol for the ex vivo generation of tracheal segments. The first step involves the application of a decellularization technique that allows for the production of a naturally derived extracellular matrix (ECM)-based bio-scaffold, that maintains the macro- and micro-architecture as well as 9 the matrix-related signals distinctive of the original tissue. In the second step chondrocytes are seeded onto decellularized trachea, using a rotating bioreactor to ensure a correct scaffold repopulation.This multi-step approach represents a powerful tool for in vitro reconstruction of a bioengineered trachea that may constitute a promising solution to restore upper airway function. In addition, the procedures here described allow for the creation of a suitable 3D platform that may find useful applications, both for toxicological studies as well as organ transplantation strategies. |
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