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Axon Regeneration

Overview of attention for book
Cover of 'Axon Regeneration'

Table of Contents

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    Book Overview
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    Chapter 7 Epigenomic Profiling of Dorsal Root Ganglia upon Regenerative and Non-regenerative Axonal Injury.
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    Chapter 15 Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish
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    Chapter 16 Translating Ribosome Affinity Purification (TRAP) and Bioinformatic RNA-Seq Analysis in Post-metamorphic Xenopus laevis.
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    Chapter 18 Profiling Dynamic Changes in DNA Accessibility During Axon Regeneration After Optic Nerve Crush in Adult Zebrafish.
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    Chapter 22 Drosophila Laser Axotomy Injury Model to Investigate RNA Repair and Splicing in Axon Regeneration
Attention for Chapter: PCNA Staining of Retinal Cryosections to Assess Microglial/Macrophage Proliferation.
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Chapter title
PCNA Staining of Retinal Cryosections to Assess Microglial/Macrophage Proliferation.
Book title
Axon Regeneration
Published in
Methods in molecular biology, January 2023
DOI 10.1007/978-1-0716-3012-9_21
Pubmed ID
36881312
Book ISBNs
978-1-07-163011-2, 978-1-07-163012-9
Authors

Lovel, Anna G, Mitchell, Diana M, Lovel, Anna G., Mitchell, Diana M.

Abstract

Detection of the protein PCNA (proliferating cell nuclear antigen) is used to identify cells in the S phase of the cell cycle to indicate cellular proliferation. Here we describe our method to detect PCNA expression by microglia and macrophages in retinal cryosections. We have used this procedure with zebrafish tissue, but this procedure could be applied to cryosections from any organism. Retinal cryosections are subjected to a heat-mediated antigen retrieval step in Citrate Buffer, then immunostained with antibodies to label PCNA and microglia/macrophages, and counterstained for cell nuclei. After fluorescent microscopy, the number of total and PCNA+ microglia/macrophages can be quantified and normalized to compare across samples and groups.

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 Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 07 March 2023.
All research outputs
#17,332,276
of 25,436,226 outputs
Outputs from Methods in molecular biology
#6,004
of 14,189 outputs
Outputs of similar age
#271,408
of 475,915 outputs
Outputs of similar age from Methods in molecular biology
#286
of 713 outputs
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