| Chapter title |
Human Leukocyte Antigen (HLA) Typing by DNA Sequencing
|
|---|---|
| Chapter number | 9 |
| Book title |
Transplantation Immunology
|
| Published in |
Methods in molecular biology, May 2013
|
| DOI | 10.1007/978-1-62703-493-7_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-62703-492-0, 978-1-62703-493-7
|
| Authors |
Ana Lazaro, Bin Tu, Ruyan Yang, Yi Xiao, Kanthi Kariyawasam, Jennifer Ng, Carolyn Katovich Hurley, Lazaro, Ana, Tu, Bin, Yang, Ruyan, Xiao, Yi, Kariyawasam, Kanthi, Ng, Jennifer, Hurley, Carolyn Katovich |
| Editors |
Andrea A. Zachary, Mary S. Leffell |
| Abstract |
DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sequencing is usually focused on the most polymorphic exons of the class I (HLA-A, -B, -C) and class II (HLA-DR, -DQ, and -DP) genes. These exons encode the antigen recognition site, the region of the HLA molecule that binds peptides and interacts with the T cell receptor for antigen and natural killer cell immunoglobulin-like receptors (KIR). Sanger sequencing of amplified DNA from each HLA gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 36 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 17% |
| Student > Master | 4 | 11% |
| Student > Ph. D. Student | 4 | 11% |
| Researcher | 3 | 8% |
| Student > Postgraduate | 2 | 6% |
| Other | 5 | 14% |
| Unknown | 12 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 28% |
| Medicine and Dentistry | 4 | 11% |
| Agricultural and Biological Sciences | 3 | 8% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
| Immunology and Microbiology | 2 | 6% |
| Other | 3 | 8% |
| Unknown | 12 | 33% |