| Chapter title |
Experimental Approaches to Study Somatic Transposition in Drosophila Using Whole-Genome DNA Sequencing.
|
|---|---|
| Chapter number | 14 |
| Book title |
Transposable Elements
|
| Published in |
Methods in molecular biology, January 2023
|
| DOI | 10.1007/978-1-0716-2883-6_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-07-162882-9, 978-1-07-162883-6
|
| Authors |
van den Beek, Marius, Rubanova, Natalia, Siudeja, Katarzyna, Beek, Marius |
| Abstract |
The extent of transposable element (TE) mobilization in different somatic tissues and throughout diverse species is not well understood. Somatic transposition is often challenging to study as it generates de novo TE insertions that represent rare genetic variants present in heterogenous tissues. Here, we describe experimental approaches that can be applied to address TE mobility in somatic tissues with the use of short- and long-read whole-genome DNA sequencing. Focusing on the analysis of the Drosophila melanogaster intestinal and head tissues, we provide instructions on how to design, perform, and validate experiments that aim at detecting somatic transposition. In addition to providing examples of protocols, this chapter intends to deliver general experimental guidelines that may be adapted to other fly tissues or to other species. |
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