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Mendeley readers
Chapter title |
Guidelines on Designing MicroRNA Sponges: From Construction to Stable Cell Line.
|
---|---|
Chapter number | 20 |
Book title |
MicroRNA Profiling
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Published in |
Methods in molecular biology, January 2017
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DOI | 10.1007/978-1-4939-6524-3_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-6522-9, 978-1-4939-6524-3
|
Authors |
Manoela Marques Ortega Ph.D., Hakim Bouamar, Manoela Marques Ortega |
Editors |
Sweta Rani |
Abstract |
Single microRNA (miRNA) can be inhibited using antagomiR which efficiently knockdown a specific miRNA. However, the effect is transient and often results in subtle phenotype. Here we report a guideline on designing miRNA sponge inhibiting a miRNA family. As a model system, we targeted miR-30 family, known as tumor suppressor miRNAs in multiple tumors. To achieve an efficient knockdown, we generated perfect and bulged-matched miRNA binding sites (MBS) and introduced multiple copies of MBS. The protocol here demonstrates the miRNA sponge as a useful tool to examine the functional impact of inhibition miRNAs. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 2 | 33% |
Researcher | 1 | 17% |
Student > Postgraduate | 1 | 17% |
Student > Master | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 50% |
Medicine and Dentistry | 1 | 17% |
Unknown | 2 | 33% |