Chapter title |
Mass Spectrometry-Based Quantitative O-GlcNAcomic Analysis.
|
---|---|
Chapter number | 6 |
Book title |
Quantitative Proteomics by Mass Spectrometry
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3524-6_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3522-2, 978-1-4939-3524-6
|
Authors |
Junfeng Ma, Gerald W. Hart Ph.D., Gerald W. Hart |
Editors |
Salvatore Sechi |
Abstract |
The dynamic co- and post-translational modification (PTM) of proteins, O-linked β-D-N-acetylglucosamine modification (O-GlcNAcylation) of serine/threonine residues is critical in many cellular processes, contributing to multiple physiological and pathological events. The term "O-GlcNAcome" refers to not only the complete set of proteins that undergo O-GlcNAcylation but also the O-GlcNAc status at individual residues, as well as the dynamics of O-GlcNAcylation in response to various stimuli. O-GlcNAcomic analyses have been a challenge for many years. In this chapter, we describe a recently developed approach for the identification and quantification of O-GlcNAc proteins/peptides from complex samples. |
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Demographic breakdown
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Researcher | 2 | 67% |
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