| Chapter title |
Tissue Engineering Platforms to Replicate the Tumor Microenvironment of Multiple Myeloma.
|
|---|---|
| Chapter number | 12 |
| Book title |
Cancer Gene Networks
|
| Published in |
Methods in molecular biology, January 2017
|
| DOI | 10.1007/978-1-4939-6539-7_12 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-6537-3, 978-1-4939-6539-7
|
| Authors |
Wenting Zhang, Woo Y. Lee, Jenny Zilberberg |
| Editors |
Usha Kasid, Robert Clarke |
| Abstract |
We described here the manufacturing and implementation of two prototype perfusion culture devices designed primarily for the cultivation of difficult-to-preserve primary patient-derived multiple myeloma cells (MMC). The first device consists of an osteoblast (OSB)-derived 3D tissue scaffold constructed in a perfused microfluidic environment. The second platform is a 96-well plate-modified perfusion culture device that can be utilized to reconstruct several tissue and tumor microenvironments utilizing both primary human and murine cells. This culture device was designed and fabricated specifically to: (1) enable the preservation of primary MMC for downstream use in biological studies and chemosensitivity analyses and, (2) provide a high-throughput format that is compatible with plate readers specifically seeing that this system is built on an industry standard 96-well tissue culture plate. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 4 | 21% |
| Researcher | 4 | 21% |
| Student > Master | 3 | 16% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 6 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 4 | 21% |
| Medicine and Dentistry | 2 | 11% |
| Materials Science | 2 | 11% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Psychology | 1 | 5% |
| Other | 3 | 16% |
| Unknown | 6 | 32% |