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Germline Stem Cells

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Cover of 'Germline Stem Cells'

Table of Contents

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    Book Overview
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    Chapter 1 Analysis of the C. elegans Germline Stem Cell Pool.
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    Chapter 2 Methods for Studying the Germline of the Human Parasite Schistosoma mansoni.
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    Chapter 3 Evaluation of the Asymmetric Division of Drosophila Male Germline Stem Cells.
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    Chapter 4 Live Imaging of the Drosophila Testis Stem Cell Niche.
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    Chapter 5 RNA Isolation from Early Drosophila Larval Ovaries.
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    Chapter 6 Live-Cell Imaging of the Adult Drosophila Ovary Using Confocal Microscopy.
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    Chapter 7 Measurement of mRNA Poly(A) Tail Lengths in Drosophila Female Germ Cells and Germ-Line Stem Cells.
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    Chapter 8 Identification of Germ-Line Stem Cells in Zebrafish.
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    Chapter 9 Primordial Germ Cell Isolation from Xenopus laevis Embryos.
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    Chapter 10 Single-Cell Lineage Analysis of Oogenesis in Mice.
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    Chapter 11 Visualization and Lineage Tracing of Pax7(+) Spermatogonial Stem Cells in the Mouse.
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    Chapter 12 Transplantation as a Quantitative Assay to Study Mammalian Male Germline Stem Cells.
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    Chapter 13 Mouse Fetal Germ Cell Isolation and Culture Techniques.
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    Chapter 14 Rattus norvegicus Spermatogenesis Colony-Forming Assays.
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    Chapter 15 Identification of Mouse piRNA Pathway Components Using Anti-MIWI2 Antibodies.
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    Chapter 16 Efficient Induction and Isolation of Human Primordial Germ Cell-Like Cells from Competent Human Pluripotent Stem Cells.
Attention for Chapter 7: Measurement of mRNA Poly(A) Tail Lengths in Drosophila Female Germ Cells and Germ-Line Stem Cells.
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Chapter title
Measurement of mRNA Poly(A) Tail Lengths in Drosophila Female Germ Cells and Germ-Line Stem Cells.
Chapter number 7
Book title
Germline Stem Cells
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-4017-2_7
Pubmed ID
27734350
Book ISBNs
978-1-4939-4015-8, 978-1-4939-4017-2
Authors

Aymeric Chartier, Willy Joly, Martine Simonelig

Editors

Michael Buszczak

Abstract

mRNA regulation by poly(A) tail length variations plays an important role in many developmental processes. Recent advances have shown that, in particular, deadenylation (the shortening of mRNA poly(A) tails) is essential for germ-line stem cell biology in the Drosophila ovary. Therefore, a rapid and accurate method to analyze poly(A) tail lengths of specific mRNAs in this tissue is valuable. Several methods of poly(A) test (PAT) assays have been reported to measure mRNA poly(A) tail lengths in vivo. Here, we describe two of these methods (PAT and ePAT) that we have adapted for Drosophila ovarian germ cells and germ-line stem cells.

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Mendeley readers

The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 10 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 3 30%
Researcher 2 20%
Student > Ph. D. Student 1 10%
Student > Bachelor 1 10%
Unknown 3 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 3 30%
Biochemistry, Genetics and Molecular Biology 1 10%
Immunology and Microbiology 1 10%
Social Sciences 1 10%
Medicine and Dentistry 1 10%
Other 0 0%
Unknown 3 30%

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