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RNA and DNA Editing

Overview of attention for book
Cover of 'RNA and DNA Editing'

Table of Contents

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    Book Overview
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    Chapter 1 Three-Dimensional Reconstruction of Trypanosoma brucei Editosomes Using Single-Particle Electron Microscopy
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    Chapter 2 iCODA: RNAi-based inducible knock-in system in Trypanosoma brucei.
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    Chapter 3 Perturbing A-to-I RNA Editing Using Genetics and Homologous Recombination
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    Chapter 4 Laser Microdissection and Pressure Catapulting of Single Human Motor Neurons for RNA Editing Analysis
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    Chapter 5 Biochemical identification of A-to-I RNA editing sites by the inosine chemical erasing (ICE) method.
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    Chapter 6 Identifying mRNA Editing Deaminase Targets by RNA-Seq
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    Chapter 7 Mouse and Other Rodent Models of C to U RNA Editing
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    Chapter 8 In Vivo Analysis of RNA Editing in Plastids
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    Chapter 9 Identifying Specific Trans -Factors of RNA Editing in Plant Mitochondria by Multiplex Single Base Extension Typing
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    Chapter 10 Complementation of Mutants in Plant Mitochondrial RNA Editing by Protoplast Transfection
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    Chapter 11 A high-throughput assay for DNA deaminases.
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    Chapter 12 Biochemical Fractionation and Purification of High-Molecular-Mass APOBEC3G Complexes
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    Chapter 13 Analysis of tRNA Editing in Native and Synthetic Substrates
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    Chapter 14 Post-transcriptional Modification of RNAs by Artificial Box H/ACA and Box C/D RNPs
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    Chapter 15 Functional Analysis of Noncoding RNAs in Trypanosomes: RNA Walk, a Novel Approach to Study RNA–RNA Interactions Between Small RNA and Its Target
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    Chapter 16 A Post-Labeling Approach for the Characterization and Quantification of RNA Modifications Based on Site-Directed Cleavage by DNAzymes
Attention for Chapter 15: Functional Analysis of Noncoding RNAs in Trypanosomes: RNA Walk, a Novel Approach to Study RNA–RNA Interactions Between Small RNA and Its Target
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Chapter title
Functional Analysis of Noncoding RNAs in Trypanosomes: RNA Walk, a Novel Approach to Study RNA–RNA Interactions Between Small RNA and Its Target
Chapter number 15
Book title
RNA and DNA Editing
Published in
Methods in molecular biology, January 2011
DOI 10.1007/978-1-61779-018-8_15
Pubmed ID
21370053
Book ISBNs
978-1-61779-017-1, 978-1-61779-018-8
Authors

Shulamit Michaeli, Chaim Wachtel

Abstract

The recent discovery of thousands of small noncoding RNAs (ncRNAs), in many different organisms, has led to the need for methods to study their function. One way to help understand their function is to determine what other RNAs interact with the ncRNAs. We have developed a novel method to investigate the RNA-RNA interactions between a small RNA and its target that we termed "RNA walk." The method is based on UV-induced AMT cross-linking in vivo followed by affinity selection of the hybrid molecules and mapping the intermolecular adducts by RT-PCR. Domains carrying the cross-linked adducts are less efficiently amplified than domains that are not cross-linked. Real-time PCR is used to quantify the results. Further mapping of the interactions is performed by primer extension to determine the exact cross-linked adduct.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
France 1 13%
Unknown 7 88%

Demographic breakdown

Readers by professional status Count As %
Professor > Associate Professor 3 38%
Student > Ph. D. Student 1 13%
Professor 1 13%
Researcher 1 13%
Student > Master 1 13%
Other 0 0%
Unknown 1 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 4 50%
Biochemistry, Genetics and Molecular Biology 3 38%
Unknown 1 13%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 February 2013.
All research outputs
#20,180,477
of 22,694,633 outputs
Outputs from Methods in molecular biology
#9,829
of 13,046 outputs
Outputs of similar age
#169,911
of 180,381 outputs
Outputs of similar age from Methods in molecular biology
#199
of 230 outputs
Altmetric has tracked 22,694,633 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,046 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 230 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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