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The Golgi Complex

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Cover of 'The Golgi Complex'

Table of Contents

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    Book Overview
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    Chapter 1 4D Confocal Imaging of Yeast Organelles
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    Chapter 2 Imaging the Polarized Sorting of Proteins from the Golgi Complex in Live Neurons
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    Chapter 3 Imaging Golgi Outposts in Fixed and Living Neurons
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    Chapter 4 Analysis of Arf1 GTPase-Dependent Membrane Binding and Remodeling Using the Exomer Secretory Vesicle Cargo Adaptor
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    Chapter 5 STEM Tomography Imaging of Hypertrophied Golgi Stacks in Mucilage-Secreting Cells
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    Chapter 6 Reconstitution of COPI Vesicle and Tubule Formation
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    Chapter 7 Reconstitution of Phospholipase A2-Dependent Golgi Membrane Tubules
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    Chapter 8 Proteomic Characterization of Golgi Membranes Enriched from Arabidopsis Suspension Cell Cultures
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    Chapter 9 High-Content Analysis of the Golgi Complex by Correlative Screening Microscopy
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    Chapter 10 Activity Detection of GalNAc Transferases by Protein-Based Fluorescence Sensors In Vivo
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    Chapter 11 In Situ Proximity Ligation Assay (PLA) Analysis of Protein Complexes Formed Between Golgi-Resident, Glycosylation-Related Transporters and Transferases in Adherent Mammalian Cell Cultures
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    Chapter 12 Creating Knockouts of Conserved Oligomeric Golgi Complex Subunits Using CRISPR-Mediated Gene Editing Paired with a Selection Strategy Based on Glycosylation Defects Associated with Impaired COG Complex Function
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    Chapter 13 Reversible Controlled Aggregation of Golgi Resident Enzymes to Assess Their Transport/Dynamics Along the Secretory Pathway
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    Chapter 14 Assays to Study the Fragmentation of the Golgi Complex During the G2–M Transition of the Cell Cycle
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    Chapter 15 The Role of Lysophospholipid Acyltransferases in the Golgi Complex
  17. Altmetric Badge
    Chapter 16 Methods to Purify and Assay Secretory Pathway Kinases
Attention for Chapter 15: The Role of Lysophospholipid Acyltransferases in the Golgi Complex
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Chapter title
The Role of Lysophospholipid Acyltransferases in the Golgi Complex
Chapter number 15
Book title
The Golgi Complex
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-6463-5_15
Pubmed ID
Book ISBNs
978-1-4939-6461-1, 978-1-4939-6463-5
Authors

John A. Schmidt

Editors

William J. Brown

Abstract

Determining the abundance of phospholipids and neutral lipids in cellular membranes is paramount to understanding their biological functions. Many lipid-modifying enzymes have yet to be characterized due to limitations in substrate-product measurements and purification of membrane-bound enzymes. The method described here uses radiolabeled phospholipid substrates and cell-purified organelles to quantify phospholipid metabolism using thin-layer chromatography. This assay has the benefits of being specific and adaptable for numerous applications and systems.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 1 100%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 100%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 February 2017.
All research outputs
#20,341,859
of 22,888,307 outputs
Outputs from Methods in molecular biology
#9,921
of 13,132 outputs
Outputs of similar age
#330,770
of 393,716 outputs
Outputs of similar age from Methods in molecular biology
#1,054
of 1,471 outputs
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