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Heterologous Expression of Membrane Proteins

Overview of attention for book
Cover of 'Heterologous Expression of Membrane Proteins'

Table of Contents

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    Book Overview
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    Chapter 1 Heterologous Expression of Membrane Proteins
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    Chapter 2 Heterologous Expression of Membrane Proteins
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    Chapter 3 Membrane Protein Production in Escherichia coli: Protocols and Rules.
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    Chapter 4 Codon Optimizing for Increased Membrane Protein Production: A Minimalist Approach.
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    Chapter 5 Generation of Tetracycline-Inducible Mammalian Cell Lines by Flow Cytometry for Improved Overproduction of Membrane Proteins.
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    Chapter 6 Membrane Protein Production in Lactococcus lactis for Functional Studies.
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    Chapter 7 Heterologous Expression of Membrane Proteins
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    Chapter 8 Leishmania tarentolae as a Promising Tool for Expressing Polytopic and Multi-Transmembrane Spans Eukaryotic Membrane Proteins: The Case of the ABC Pump ABCG6.
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    Chapter 9 Overexpression, Membrane Preparation, and Purification of a Typical Multidrug ABC Transporter BmrA.
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    Chapter 10 Heterologous Expression of Membrane Proteins
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    Chapter 11 Heterologous Expression of Membrane Proteins
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    Chapter 12 Heterologous Expression of Membrane Proteins
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    Chapter 13 Heterologous Expression of Membrane Proteins
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    Chapter 14 Heterologous Expression of Membrane Proteins
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    Chapter 15 Membrane Protein Solubilization and Composition of Protein Detergent Complexes.
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    Chapter 16 Detergent-Free Membrane Protein Purification.
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    Chapter 17 Conformational Dynamics and Interactions of Membrane Proteins by Hydrogen/Deuterium Mass Spectrometry.
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    Chapter 18 Lessons from an α-Helical Membrane Enzyme: Expression, Purification, and Detergent Optimization for Biophysical and Structural Characterization.
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    Chapter 19 Heterologous Expression of Membrane Proteins
Attention for Chapter 12: Heterologous Expression of Membrane Proteins
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (53rd percentile)
  • Good Attention Score compared to outputs of the same age and source (77th percentile)

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Chapter title
Heterologous Expression of Membrane Proteins
Chapter number 12
Book title
Heterologous Expression of Membrane Proteins
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3637-3_12
Pubmed ID
Book ISBNs
978-1-4939-3635-9, 978-1-4939-3637-3
Authors

Kalathur, Ravi C, Panganiban, Marinela, Bruni, Renato, Ravi C. Kalathur, Marinela Panganiban, Renato Bruni

Editors

Isabelle Mus-Veteau

Abstract

The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a high-throughput manner.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 50%
Student > Ph. D. Student 2 25%
Professor > Associate Professor 1 13%
Student > Postgraduate 1 13%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 50%
Pharmacology, Toxicology and Pharmaceutical Science 1 13%
Agricultural and Biological Sciences 1 13%
Psychology 1 13%
Neuroscience 1 13%
Other 0 0%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 December 2017.
All research outputs
#12,963,262
of 22,882,389 outputs
Outputs from Methods in molecular biology
#3,295
of 13,131 outputs
Outputs of similar age
#179,434
of 393,707 outputs
Outputs of similar age from Methods in molecular biology
#310
of 1,471 outputs
Altmetric has tracked 22,882,389 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one has gotten more attention than average, scoring higher than 74% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,707 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 53% of its contemporaries.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 77% of its contemporaries.