↓ Skip to main content
What is this page? Embed badge

Induced Pluripotent Stem (iPS) Cells

Overview of attention for book
Cover of 'Induced Pluripotent Stem (iPS) Cells'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 299 The Characteristics of Human iPS Cells and siRNA Transfection Under Hypoxia
  3. Altmetric Badge
    Chapter 300 Generation of Quiescent Cardiac Fibroblasts Derived from Human Induced Pluripotent Stem Cells
  4. Altmetric Badge
    Chapter 301 Pluripotent Stem Cell Differentiation Toward Functional Basal Stratified Epithelial Cells
  5. Altmetric Badge
    Chapter 302 mRNA-Based Reprogramming Under Xeno-Free and Feeder-Free Conditions
  6. Altmetric Badge
    Chapter 303 Embryonal Carcinoma and Glioblastoma Cell Lines Derived from Monkey Induced Pluripotent Stem Cells
  7. Altmetric Badge
    Chapter 304 Generation of Monoclonal iPSC Lines with Stable Cas9 Expression and High Cas9 Activity
  8. Altmetric Badge
    Chapter 318 Robust and Highly Efficient Protocol for Differentiation of Human Pluripotent Stem Cells into Mesenchymal Stem Cells
  9. Altmetric Badge
    Chapter 323 Highly Efficient Differentiation of Human Pluripotent Stem Cells into Pancreatic Progenitors Co-expressing PDX1 and NKX6.1
  10. Altmetric Badge
    Chapter 327 Derivation and Characterization of Mesenchymal Stem Cells from iPS Cells
  11. Altmetric Badge
    Chapter 328 Isolation and Culture of Human-Induced Pluripotent Stem Cell-Derived Cerebral Organoid Cells
  12. Altmetric Badge
    Chapter 334 Production of Innervated Skeletal Muscle Fibers Using Human Induced Pluripotent Stem Cells
  13. Altmetric Badge
    Chapter 335 Inductive Coculture Differentiation of Induced Pluripotent Stem Cells into Cardiomyocytes
  14. Altmetric Badge
    Chapter 336 3D Microwell Platform for Cardiomyocyte Differentiation of Human Pluripotent Stem Cells
  15. Altmetric Badge
    Chapter 337 CRISPR/Cas9–Mediated Gene Knockout and Knockin Human iPSCs
  16. Altmetric Badge
    Chapter 348 Gene Editing in Human Induced Pluripotent Stem Cells Using Doxycycline-Inducible CRISPR-Cas9 System
  17. Altmetric Badge
    Chapter 349 Derivation of Clinical-Grade Induced Pluripotent Stem Cell Lines from Erythroid Progenitor Cells in Xenofree Conditions
  18. Altmetric Badge
    Chapter 350 Generation of Murine Induced Pluripotent Stem Cells through Transposon-Mediated Reprogramming
  19. Altmetric Badge
    Chapter 351 The Development of Tissue Engineering Scaffolds Using Matrix from iPS-Reprogrammed Fibroblasts
  20. Altmetric Badge
    Chapter 352 CRISPR/Cas9 Ribonucleoprotein Complex-Mediated Efficient B2M Knockout in Human Induced Pluripotent Stem Cells (iPSCs)
  21. Altmetric Badge
    Chapter 353 Efficient and Safe Method of Generating Induced Pluripotent Stem Cells from Human Skin Fibroblasts and Subsequent Differentiation into Functional Cardiomyocytes
  22. Altmetric Badge
    Chapter 354 Detecting and Modulating ER Stress to Improve Generation of Induced Pluripotent Stem Cells
  23. Altmetric Badge
    Chapter 355 Efficient Generation of iPSC-Derived Hematoendothelial Progenitors and Specification Toward T cell Lineage
  24. Altmetric Badge
    Chapter 356 A Method for Encapsulation and Transplantation into Diabetic Mice of Human Induced Pluripotent Stem Cells (hiPSC)-Derived Pancreatic Progenitors
  25. Altmetric Badge
    Chapter 357 3D Organoid Culture Using Skin Keratinocytes Derived from Human Induced Pluripotent Stem Cells
  26. Altmetric Badge
    Chapter 358 Human Induced Pluripotent Stem Cell (iPSC) Handling Protocols: Maintenance, Expansion, and Cryopreservation
  27. Altmetric Badge
    Chapter 359 Human Pluripotent Stem Cell Differentiation to Microglia
  28. Altmetric Badge
    Chapter 360 A Simple Method for Generating, Clearing, and Imaging Pre-vascularized 3D Adipospheres Derived from Human iPS Cells
  29. Altmetric Badge
    Chapter 361 A Method for In Vitro Fabrication of Hybrid Bone/Cartilage Tissue Using Mouse Induced Pluripotent Stem Cells
  30. Altmetric Badge
    Chapter 362 CRISPR/Cas9-Mediated Genome Editing to Generate Clonal iPSC Lines
  31. Altmetric Badge
    Chapter 363 Differentiation of Human Induced Pluripotent Stem Cells (hiPSC) into Endothelial-Type Cells and Establishment of an In Vitro Blood-Brain Barrier Model
  32. Altmetric Badge
    Chapter 364 Non-modified RNA-Based Reprogramming of Human Dermal Fibroblasts into Induced Pluripotent Stem Cells
  33. Altmetric Badge
    Chapter 368 CRISPR/Cas9-Mediated Introduction of Specific Heterozygous Mutations in Human Induced Pluripotent Stem Cells
  34. Altmetric Badge
    Chapter 369 Application of Human Induced Pluripotent Stem Cell Technology for Cardiovascular Regenerative Pharmacology
  35. Altmetric Badge
    Chapter 370 The Differentiation of Human Induced Pluripotent Stem Cells into Podocytes In Vitro
  36. Altmetric Badge
    Chapter 372 Generation of Human Induced Pluripotent Stem Cells Using Endothelial Progenitor Cells Derived from Umbilical Cord Blood and Adult Peripheral Blood
  37. Altmetric Badge
    Chapter 373 Efficient Induction of Primate iPS Cells Using a Combination of RNA Transfection and Chemical Compounds
  38. Altmetric Badge
    Chapter 380 Generation and Cultivation of Transgene-Free Macaque and Baboon iPSCs Under Chemically Defined Conditions
  39. Altmetric Badge
    Chapter 381 Generation of Marmoset Monkey iPSCs with Self-Replicating VEE-mRNAs in Feeder-Free Conditions
  40. Altmetric Badge
    Chapter 382 An Optical-Flow-Based Method to Quantify Dynamic Behavior of Human Pluripotent Stem Cell-Derived Cardiomyocytes in Disease Modeling Platforms.
  41. Altmetric Badge
    Chapter 386 Generation of Human Neural Progenitors from Blood Samples by Interrupted Reprogramming
  42. Altmetric Badge
    Chapter 387 Methods for Isolation and Reprogramming of Various Somatic Cell Sources into iPSCs
  43. Altmetric Badge
    Chapter 388 Differentiation of Human Induced Pluripotent Stem Cells into Definitive Endoderm Using Simple Dialysis Culture Device
  44. Altmetric Badge
    Chapter 391 Efficient High-Density hiPSCs Expansion in Simple Dialysis Device
  45. Altmetric Badge
    Chapter 392 Derivation of Three-Dimensional Human Induced Pluripotent Stem Cell-Derived Vocal Fold Mucosa for Clinical and Pharmacological Applications
  46. Altmetric Badge
    Chapter 393 Development of a Blood–Brain Barrier Permeability Assay Using Human Induced Pluripotent Stem Cell Derived Brain Endothelial Cells
  47. Altmetric Badge
    Chapter 394 Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
  48. Altmetric Badge
    Chapter 395 Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
  49. Altmetric Badge
    Chapter 408 Expanding the Differentiation Potential of Already-Established Pluripotent Stem Cells
  50. Altmetric Badge
    Chapter 414 Analysis of Clonal Composition in Human iPSC and ESC and Derived 2D and 3D Differentiated Cultures.
  51. Altmetric Badge
    Chapter 428 Culturing Human Pluripotent Stem Cells on Micropatterned Silicon Surfaces
  52. Altmetric Badge
    Chapter 429 Human Induced Pluripotent Stem Cell-Derived Microglia (hiPSC-Microglia).
  53. Altmetric Badge
    Chapter 430 Serum-Free Production of Three-Dimensional Hepatospheres from Pluripotent Stem Cells
  54. Altmetric Badge
    Chapter 443 Revised “hPSC-Sac Method” for Simple and Efficient Differentiation of Human Pluripotent Stem Cells to Hematopoietic Progenitor Cells
  55. Altmetric Badge
    Chapter 445 Generation of Induced Pluripotent Stem Cells from Human Bone Marrow–Derived Mesenchymal Stem Cells
  56. Altmetric Badge
    Chapter 446 Porcine iPSC Generation: Testing Different Protocols to a Successful Application
  57. Altmetric Badge
    Chapter 458 Correction to: Human Pluripotent Stem Cells for High-Throughput Drug Screening and Characterization of Small Molecules
Attention for Chapter 395: Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
1 X user

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
7 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Scalable Generation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes.
Chapter number 395
Book title
Induced Pluripotent Stem (iPS) Cells
Published in
Methods in molecular biology, October 2021
DOI 10.1007/7651_2021_395
Pubmed ID
34664217
Book ISBNs
978-1-07-162118-9, 978-1-07-162119-6
Authors

Hamad, Sarkawt, Derichsweiler, Daniel, Hescheler, Jürgen, Pfannkuche, Kurt

Abstract

Human induced pluripotent stem cells (hiPSCs) can be expanded at limitless scale in vitro and give rise to various organotypic cells, cardiomyocytes (CMs) among them. Advanced protocols shape the differentiation process of pluripotent stem cells by controlled growth factor application. Modulating the Wnt signaling pathway is effective to direct hiPSCs to CMs (hiPSC-CMs) and native growth factors were replaced by small chemical compounds. Here, we describe a refined protocol for scalable generation of hiPSC-CMs that manipulates porcupine and tankyrase sub-pathways of Wnt signaling for tight inhibition of non-canonical Wnt signaling. The approach results in a differentiation efficiency toward hiPSC-CMs of 87 ± 0.9% in stirred bioreactor cultures and yields about 70 million hiPSC-CMs per 100 mL serum free cardiac differentiation medium. The differentiation protocol is easily adapted from 3D to 2D culture and vice versa and has been demonstrated to work with different hiPSC lines.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 7 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 7 100%

Demographic breakdown

Readers by professional status Count As %
Professor 1 14%
Researcher 1 14%
Unknown 5 71%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 1 14%
Chemistry 1 14%
Medicine and Dentistry 1 14%
Unknown 4 57%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2021.
All research outputs
#15,686,478
of 23,310,485 outputs
Outputs from Methods in molecular biology
#5,497
of 13,324 outputs
Outputs of similar age
#250,059
of 439,284 outputs
Outputs of similar age from Methods in molecular biology
#100
of 301 outputs
Altmetric has tracked 23,310,485 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,324 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 439,284 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 301 others from the same source and published within six weeks on either side of this one. This one is in the 49th percentile – i.e., 49% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.