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Bioreactors in Stem Cell Biology

Overview of attention for book
Cover of 'Bioreactors in Stem Cell Biology'

Table of Contents

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    Book Overview
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    Chapter 396 Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors
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    Chapter 397 Fabrication Protocol for Thermoplastic Microfluidic Devices: Nanoliter Volume Bioreactors for Cell Culturing
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    Chapter 398 Tracheal In Vitro Reconstruction Using a Decellularized Bio-Scaffold in Combination with a Rotating Bioreactor.
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    Chapter 410 Optimized Method to Improve Cell Activity in 3D Scaffolds Under a Dual Real-Time Dynamic Bioreactor System
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    Chapter 411 Microcarrier-Supported Culture of Chondrocytes in Continuously Rocked Disposable Bioreactor
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    Chapter 412 High-Efficiency Differentiation of Human Pluripotent Stem Cells to Hematopoietic Stem/Progenitor Cells in Random Positioning Machine Bioreactors
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    Chapter 415 Volumetric Mass Transfer Coefficient Measurement in a Stirred Tank Reactor
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    Chapter 416 Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors.
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    Chapter 417 Bacterial Nanocellulose-Based Grafts for Cell Colonization Studies: An In Vitro Bioreactor Perfusion Model
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    Chapter 423 Integrating Human-Induced Pluripotent Stem Cell Expansion Capability and Cardiomyocyte Differentiation Potential in a Microcarrier Suspension Culture
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    Chapter 424 A Guideline to Set Up Cascaded Continuous Cultivation with E. coli Bl21 (DE3)
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    Chapter 425 Large-Scale Expansion of Umbilical Cord Mesenchymal Stem Cells with Microcarrier Tablets in Bioreactor
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    Chapter 426 Chemically Defined, Xeno-Free Expansion of Human Mesenchymal Stem Cells (hMSCs) on Benchtop-Scale Using a Stirred Single-Use Bioreactor
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    Chapter 431 Bioreactor-Based De-epithelialization of Long-Segment Tracheal Grafts
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    Chapter 432 In Vitro 3D Mechanical Stimulation to Tendon-Derived Stem Cells by Bioreactor
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    Chapter 441 Benchtop Bioreactors in Mammalian Cell Culture: Overview and Guidelines
  18. Altmetric Badge
    Chapter 444 Bioreactor-Based Adherent Cells Harvesting from Microcarriers with 3D Printed Inertial Microfluidics
Attention for Chapter 416: Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors.
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Chapter title
Extracellular Vesicle Collection from Human Stem Cells Grown in Suspension Bioreactors.
Chapter number 416
Book title
Bioreactors in Stem Cell Biology
Published in
Methods in molecular biology, September 2021
DOI 10.1007/7651_2021_416
Pubmed ID
34490594
Book ISBNs
978-1-07-162017-5, 978-1-07-162018-2
Authors

Yuan, Xuegang, Chen, Xingchi, Zeng, Changchun, Meckes, David G, Li, Yan, Meckes, David G.

Abstract

Extracellular vesicles (EVs) are particles with 100-1000 nm sizes which are secreted by cells for intercellular communication. Meanwhile, studies have found that EVs secreted by human stem cells carry similar characteristics (microRNAs, proteins, metabolites, etc.) from their cell counterpart. Thus, EVs derived from stem cells, especially human induced pluripotent stem cells (hiPSCs) and human mesenchymal stromal/stem cells (hMSCs) are promising candidates for cell-free therapy. However, conventional planar culture is insufficient to produce a large amount of cells or EVs to satisfy clinical requirements. In this chapter, we described feasible approaches to harvest EVs secreted by lineage-specific hiPSCs and undifferentiated hMSCs in suspension bioreactors. Differentiation of hiPSCs to cortical organoids can be performed in suspension bioreactors and the corresponding EVs can be isolated and purified. This scale-up protocol can be applied to a majority of stem cell types with EV collection thus provides useful information for both experimental and biomanufacturing purposes.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 13%
Student > Bachelor 1 13%
Researcher 1 13%
Unknown 5 63%
Readers by discipline Count As %
Chemical Engineering 1 13%
Economics, Econometrics and Finance 1 13%
Engineering 1 13%
Unknown 5 63%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 September 2021.
All research outputs
#20,710,927
of 23,310,485 outputs
Outputs from Methods in molecular biology
#10,098
of 13,324 outputs
Outputs of similar age
#356,073
of 429,637 outputs
Outputs of similar age from Methods in molecular biology
#202
of 272 outputs
Altmetric has tracked 23,310,485 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,324 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 272 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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