| Chapter title |
Low-Cost Peptide Microarrays for Mapping Continuous Antibody Epitopes
|
|---|---|
| Chapter number | 6 |
| Book title |
Peptide Microarrays
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3037-1_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3036-4, 978-1-4939-3037-1
|
| Authors |
Ryan McBride, Steven R. Head, Phillip Ordoukhanian, Mansun Law |
| Editors |
Marina Cretich, Marcella Chiari |
| Abstract |
With the increasing need for understanding antibody specificity in antibody and vaccine research, pepscan assays provide a rapid method for mapping and profiling antibody responses to continuous epitopes. We have developed a relatively low-cost method to generate peptide microarray slides for studying antibody binding. Using a setup of an IntavisAG MultiPep RS peptide synthesizer, a Digilab MicroGrid II 600 microarray printer robot, and an InnoScan 1100 AL scanner, the method allows the interrogation of up to 1536 overlapping, alanine-scanning, and mutant peptides derived from the target antigens. Each peptide is tagged with a polyethylene glycol aminooxy terminus to improve peptide solubility, orientation, and conjugation efficiency to the slide surface. |
X Demographics
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|---|---|---|
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Demographic breakdown
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|---|---|---|
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Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 7 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 2 | 29% |
| Student > Ph. D. Student | 1 | 14% |
| Student > Postgraduate | 1 | 14% |
| Unknown | 3 | 43% |
| Readers by discipline | Count | As % |
|---|---|---|
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| Unknown | 3 | 43% |