Chapter title |
Structures and Analysis of Carotenoid Molecules.
|
---|---|
Chapter number | 3 |
Book title |
Carotenoids in Nature
|
Published in |
Sub cellular biochemistry, August 2016
|
DOI | 10.1007/978-3-319-39126-7_3 |
Pubmed ID | |
Book ISBNs |
978-3-31-939124-3, 978-3-31-939126-7
|
Authors |
Delia B. Rodriguez-Amaya |
Editors |
Claudia Stange |
Abstract |
Modifications of the usual C40 linear and symmetrical carotenoid skeleton give rise to a wide array of structures of carotenes and xanthophylls in plant tissues. These include acyclic, monocyclic and dicyclic carotenoids, along with hydroxy and epoxy xanthophylls and apocarotenoids. Carotenols can be unesterified or esterified (monoester) in one or two (diester) hydroxyl groups with fatty acids. E-Z isomerization increases the array of possible plant carotenoids even further. Screening and especially quantitative analysis are being carried out worldwide. Visible absorption spectrometry and near infrared reflectance spectroscopy have been used for the initial estimation of the total carotenoid content or the principal carotenoid content when large numbers of samples needed to be analyzed within a short time, as would be the case in breeding programs. Although inherently difficult, quantitative analysis of the individual carotenoids is essential. Knowledge of the sources of errors and means to avoid them has led to a large body of reliable quantitative compositional data on carotenoids. Reverse-phase HPLC with a photodiode array detector has been the preferred analytical technique, but UHPLC is increasingly employed. HPLC-MS has been used mainly for identification and NMR has been useful in unequivocally identifying geometric isomers. |
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