| Chapter title |
Heterologous Expression of Membrane Proteins
|
|---|---|
| Chapter number | 10 |
| Book title |
Heterologous Expression of Membrane Proteins
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3637-3_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3635-9, 978-1-4939-3637-3
|
| Authors |
Hartmann, Lucie, Kugler, Valérie, Wagner, Renaud, Lucie Hartmann, Valérie Kugler, Renaud Wagner |
| Editors |
Isabelle Mus-Veteau |
| Abstract |
A key point when it comes to heterologous expression of eukaryotic membrane proteins (EMPs) is the choice of the best-suited expression platform. The yeast Pichia pastoris has proven to be a very versatile system showing promising results in a growing number of cases. Indeed, its particular methylotrophic characteristics combined to the very simple handling of a eukaryotic microorganism that possesses the majority of mammalian-like machineries make it a very competitive expression system for various complex proteins, in amounts compatible with functional and structural studies. This chapter describes a set of robust methodologies routinely used for the successful expression of a variety of EMPs, going from yeast transformation with the recombinant plasmid to the analysis of the quality and quantity of the proteins produced. |
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