Chapter title |
Marine Genomics
|
---|---|
Chapter number | 12 |
Book title |
Marine Genomics
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3774-5_12 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3772-1, 978-1-4939-3774-5
|
Authors |
Fonseca, Vera G, Lallias, Delphine, Vera G. Fonseca, Delphine Lallias |
Editors |
Sarah J. Bourlat |
Abstract |
The accurate assessment of community composition and ultimately species identification is of utmost importance in any ecological and evolutionary study. Advances in sequencing technologies have allowed the unraveling of levels of biodiversity never imagined before when applied to large-scale environmental DNA studies (also termed metabarcoding/metagenetics/metasystematics/environmental barcoding). Here, we describe a detailed protocol to assess eukaryotic biodiversity in marine sediments, identifying key steps that should not be neglected when preparing Next-Generation Sequencing (NGS) amplicon libraries: DNA extraction, multiple PCR amplification of DNA barcode markers with index/ tag-primers, and final Illumina MiSeq sequencing library preparation. |
X Demographics
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Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 3% |
Germany | 1 | 3% |
Unknown | 28 | 93% |
Demographic breakdown
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Student > Ph. D. Student | 7 | 23% |
Researcher | 7 | 23% |
Other | 4 | 13% |
Student > Master | 4 | 13% |
Student > Bachelor | 2 | 7% |
Other | 2 | 7% |
Unknown | 4 | 13% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 13 | 43% |
Environmental Science | 5 | 17% |
Biochemistry, Genetics and Molecular Biology | 5 | 17% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Unknown | 6 | 20% |