Chapter title |
Saturation of the secretory pathway by overexpression of a hookworm (Necator americanus) Protein (Na-ASP1).
|
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Chapter number | 5 |
Book title |
Pichia Protocols
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Published in |
Methods in molecular biology, January 2007
|
DOI | 10.1007/978-1-59745-456-8_5 |
Pubmed ID | |
Book ISBNs |
978-1-58829-429-6, 978-1-59745-456-8
|
Authors |
Inan, Mehmet, Fanders, Sarah A, Zhang, Wenhui, Hotez, Peter J, Zhan, Bin, Meagher, Michael M, Mehmet Inan, Sarah A. Fanders, Wenhui Zhang, Peter J. Hotez, Bin Zhan, Michael M. Meagher, Fanders, Sarah A., Hotez, Peter J., Meagher, Michael M. |
Abstract |
Human hookworm infection is one of the most significant parasitic infections, and a leading global cause of anemia and malnutrition of adults and children in rural areas of the tropics and subtropics. Necator americanus secretory protein (Na-ASP1), which is a potential vaccine candidate against hookworm infections, has been expressed in Pichia pastoris. Na-ASP1 protein was expressed extracellulary by employing the leader sequence of the alpha-mating factor of Saccharomyces cerevisiae. Most of the protein produced by single copy clones was secreted outside the cell. The Na-ASP1 steady state mRNA levels of the clones were correlated to their Na-ASP1 gene copy number. However, increasing gene copy number of Na-ASP1 protein in P. pastoris saturated secretory capacity and therefore, decreased the amount of secreted protein in clones harboring multiple copies of Na-ASP1 gene. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 28 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 21% |
Researcher | 5 | 18% |
Student > Bachelor | 4 | 14% |
Student > Doctoral Student | 2 | 7% |
Student > Master | 2 | 7% |
Other | 2 | 7% |
Unknown | 7 | 25% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 2 | 7% |
Chemical Engineering | 1 | 4% |
Business, Management and Accounting | 1 | 4% |
Environmental Science | 1 | 4% |
Other | 2 | 7% |
Unknown | 7 | 25% |