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Genomic Imprinting

Overview of attention for book
Cover of 'Genomic Imprinting'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Uniparental Embryos in the Study of Genomic Imprinting
  3. Altmetric Badge
    Chapter 2 Derivation of induced pluripotent stem cells by retroviral gene transduction in Mammalian species.
  4. Altmetric Badge
    Chapter 3 Generation of Trophoblast Stem Cells
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    Chapter 4 Immunomagnetic Purification of Murine Primordial Germ Cells
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    Chapter 5 Whole Genome Methylation Profiling by Immunoprecipitation of Methylated DNA
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    Chapter 6 Identification of Imprinted Loci by Transcriptome Sequencing
  8. Altmetric Badge
    Chapter 7 Data Mining as a Discovery Tool for Imprinted Genes
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    Chapter 8 Engineering of Large Deletions and Duplications In Vivo
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    Chapter 9 Methylated DNA Immunoprecipitation (MeDIP) from Low Amounts of Cells
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    Chapter 10 Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains.
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    Chapter 11 Quantitative Chromosome Conformation Capture
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    Chapter 12 Genome-wide analysis of DNA methylation in low cell numbers by reduced representation bisulfite sequencing.
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    Chapter 13 Isolation of RNA and DNA from Single Preimplantation Embryos and a Small Number of Mammalian Oocytes for Imprinting Studies
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    Chapter 14 Generation of cDNA Libraries from RNP-Derived Regulatory Noncoding RNAs
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    Chapter 15 Co-Immunoprecipitation of Long Noncoding RNAs
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    Chapter 16 Specialized technologies for epigenetics in plants.
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    Chapter 17 Computational Studies of Imprinted Genes
  19. Altmetric Badge
    Chapter 18 Insights on imprinting from beyond mice and men.
  20. Altmetric Badge
    Chapter 19 Nonmammalian Parent-of-Origin Effects
Attention for Chapter 10: Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains.
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Chapter title
Chromatin immunoprecipitation to characterize the epigenetic profiles of imprinted domains.
Chapter number 10
Book title
Genomic Imprinting
Published in
Methods in molecular biology, January 2012
DOI 10.1007/978-1-62703-011-3_10
Pubmed ID
22907496
Book ISBNs
978-1-62703-010-6, 978-1-62703-011-3
Authors

Purnima Singh, Piroska E. Szabó, Singh, Purnima, Szabó, Piroska E.

Abstract

Imprinted genes are marked by parental allele specific DNA methylation and histone modifications which regulate their monoallelic expression. Chromatin immunoprecipitation (ChIP) is the technique of choice to characterize the histones associated with either maternal or paternal chromosomes. To study allele-specific chromatin composition at imprinted regions, the method has to be efficient to work on limiting amount of starting material, and specific enough to recognize one of the parental alleles. We optimized the commonly used ChIP technique for efficient recovery of one parental allele from small number of cells. We provide examples to show that this ChIP protocol can specifically distinguish between parental alleles in mouse embryo fibroblasts carrying maternal and paternal duplication of mouse distal Chr7 and also in normal mouse embryo fibroblasts carrying single nucleotide polymorphism at imprinted regions.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 15 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 27%
Student > Master 4 27%
Researcher 3 20%
Student > Bachelor 1 7%
Unknown 3 20%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 33%
Biochemistry, Genetics and Molecular Biology 2 13%
Pharmacology, Toxicology and Pharmaceutical Science 1 7%
Immunology and Microbiology 1 7%
Social Sciences 1 7%
Other 1 7%
Unknown 4 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 August 2012.
All research outputs
#18,313,878
of 22,675,759 outputs
Outputs from Methods in molecular biology
#7,828
of 13,037 outputs
Outputs of similar age
#195,972
of 244,088 outputs
Outputs of similar age from Methods in molecular biology
#325
of 473 outputs
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So far Altmetric has tracked 13,037 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 24th percentile – i.e., 24% of its peers scored the same or lower than it.
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We're also able to compare this research output to 473 others from the same source and published within six weeks on either side of this one. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.

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