Astrocytes
Humana Press
Chapter title |
Isolation and culture of astrocytes from the retina and optic nerve.
|
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Chapter number | 8 |
Book title |
Astrocytes
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Published in |
Methods in molecular biology, December 2011
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DOI | 10.1007/978-1-61779-452-0_8 |
Pubmed ID | |
Book ISBNs |
978-1-61779-451-3, 978-1-61779-452-0
|
Authors |
Thomas J. Lukas, Ai Ling Wang |
Abstract |
Detailed methods for the preparation of optic nerve head (ONH) astrocytes from human donor eyes and retinal astrocytes from rat eyes are described. Included is the immunopanning method used for ONH astrocyte isolation as well as cell characterization. The isolation of purified retinal astrocytes is outlined as a method applicable to rodent and other mammalian retinas. |
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