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Endocannabinoid Signaling

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Cover of 'Endocannabinoid Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 Need for Methods to Investigate Endocannabinoid Signaling
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    Chapter 2 Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
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    Chapter 3 Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS
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    Chapter 4 Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices
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    Chapter 5 Assay of CB1 Receptor Binding
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    Chapter 6 The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 7 Endocannabinoid Signaling
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    Chapter 8 A Functional Assay for GPR55: Envision Protocol
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    Chapter 9 The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 10 Assay of GTPγS Binding in Autoradiography
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    Chapter 11 Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors
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    Chapter 12 Assay of NAT Activity
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    Chapter 13 Assay of NAPE-PLD Activity
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    Chapter 14 Assay of FAAH Activity
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    Chapter 15 Assay of NAAA Activity
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    Chapter 16 Assay of DAGLα/β Activity
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    Chapter 17 Endocannabinoid Signaling
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    Chapter 18 A Sensitive and Versatile Fluorescent Activity Assay for ABHD6
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    Chapter 19 A Sensitive and Versatile Fluorescent Activity Assay for ABHD12
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    Chapter 20 Assay of Endocannabinoid Uptake
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    Chapter 21 Assay of Endocannabinoid Oxidation by Cyclooxygenase-2
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    Chapter 22 Oxygenation of Anandamide by Lipoxygenases
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    Chapter 23 Assay of Endocannabinoid Oxidation by Cytochrome P450
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    Chapter 24 Assessing Gene Expression of the Endocannabinoid System
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    Chapter 25 Western Blotting of the Endocannabinoid System
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    Chapter 26 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
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    Chapter 27 Measuring ECS Interaction with Biomembranes
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    Chapter 28 Visualization of Endocannabinoids in the Cell
Attention for Chapter 28: Visualization of Endocannabinoids in the Cell
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Chapter title
Visualization of Endocannabinoids in the Cell
Chapter number 28
Book title
Endocannabinoid Signaling
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3539-0_28
Pubmed ID
Book ISBNs
978-1-4939-3537-6, 978-1-4939-3539-0
Authors

Sergio Oddi, Antonio Totaro, Mauro Maccarrone

Editors

Mauro Maccarrone

Abstract

A still unsolved, although critical, issue in endocannabinoid research is the mechanism by which the lipophilic compound anandamide (AEA) moves from its site of synthesis, crosses the aqueous milieu, and reaches the different intracellular compartments, where its metabolic and signaling pathways take place. The difficulty of studying intracellular AEA transport and distribution results from the lack of specific probes and techniques to track and visualize this bioactive lipid within the cell. Here, we describe the use of a biotinylated, non-hydrolyzable derivative of AEA (biotin-AEA, b-AEA) for visualizing the subcellular distribution of this endocannabinoid by means of confocal fluorescence microscopy.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 12 100%

Demographic breakdown

Readers by professional status Count As %
Other 4 33%
Student > Ph. D. Student 2 17%
Researcher 2 17%
Student > Master 1 8%
Professor 1 8%
Other 0 0%
Unknown 2 17%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 50%
Nursing and Health Professions 1 8%
Agricultural and Biological Sciences 1 8%
Medicine and Dentistry 1 8%
Materials Science 1 8%
Other 0 0%
Unknown 2 17%