Chapter title |
Measuring ECS Interaction with Biomembranes
|
---|---|
Chapter number | 27 |
Book title |
Endocannabinoid Signaling
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3539-0_27 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3537-6, 978-1-4939-3539-0
|
Authors |
Clotilde B. Angelucci, Annalaura Sabatucci, Enrico Dainese |
Editors |
Mauro Maccarrone |
Abstract |
Understanding the correct interaction among the different components of the endocannabinoid system (ECS) is fundamental for a proper assessment of the function of endocannabinoids (eCBs) as signaling molecules. The knowledge of how membrane environment is able to modulate intracellular trafficking of eCBs and their interacting proteins holds a huge potential in unraveling new mechanisms of ECS modulation.Here, fluorescence resonance energy transfer (FRET) technique is applied to measure the binding affinity of ECS proteins to model membranes (i.e., large unilamellar vesicles, LUVs). In particular, we describe in details the paradigmatic example of the interaction of recombinant rat FAAH-ΔTM with LUVs constituted by 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC). |
Mendeley readers
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Other | 2 | 67% |
Unknown | 1 | 33% |
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Biochemistry, Genetics and Molecular Biology | 1 | 33% |
Unknown | 2 | 67% |