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Mendeley readers
Chapter title |
Assay of NAAA Activity
|
---|---|
Chapter number | 15 |
Book title |
Endocannabinoid Signaling
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3539-0_15 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3537-6, 978-1-4939-3539-0
|
Authors |
Kazuhito Tsuboi, Natsuo Ueda |
Editors |
Mauro Maccarrone |
Abstract |
N-acylethanolamine-hydrolyzing acid amidase (NAAA) is a lysosomal hydrolase degrading various N-acylethanolamines at acidic pH. Since NAAA prefers anti-inflammatory and analgesic palmitoylethanolamide to other N-acylethanolamines as a substrate, its specific inhibitors are expected as a new class of anti-inflammatory and analgesic agents. Here, we introduce an NAAA assay system, using [(14)C]palmitoylethanolamide and thin-layer chromatography. The preparation of NAAA enzyme from native and recombinant sources as well as the chemical synthesis of N-[1'-(14)C]palmitoyl-ethanolamine is also described. |
Mendeley readers
The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 1 | 100% |
Readers by discipline | Count | As % |
---|---|---|
Unknown | 1 | 100% |