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Endocannabinoid Signaling

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Cover of 'Endocannabinoid Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 Need for Methods to Investigate Endocannabinoid Signaling
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    Chapter 2 Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
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    Chapter 3 Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS
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    Chapter 4 Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices
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    Chapter 5 Assay of CB1 Receptor Binding
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    Chapter 6 The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 7 Endocannabinoid Signaling
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    Chapter 8 A Functional Assay for GPR55: Envision Protocol
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    Chapter 9 The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 10 Assay of GTPγS Binding in Autoradiography
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    Chapter 11 Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors
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    Chapter 12 Assay of NAT Activity
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    Chapter 13 Assay of NAPE-PLD Activity
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    Chapter 14 Assay of FAAH Activity
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    Chapter 15 Assay of NAAA Activity
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    Chapter 16 Assay of DAGLα/β Activity
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    Chapter 17 Endocannabinoid Signaling
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    Chapter 18 A Sensitive and Versatile Fluorescent Activity Assay for ABHD6
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    Chapter 19 A Sensitive and Versatile Fluorescent Activity Assay for ABHD12
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    Chapter 20 Assay of Endocannabinoid Uptake
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    Chapter 21 Assay of Endocannabinoid Oxidation by Cyclooxygenase-2
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    Chapter 22 Oxygenation of Anandamide by Lipoxygenases
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    Chapter 23 Assay of Endocannabinoid Oxidation by Cytochrome P450
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    Chapter 24 Assessing Gene Expression of the Endocannabinoid System
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    Chapter 25 Western Blotting of the Endocannabinoid System
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    Chapter 26 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
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    Chapter 27 Measuring ECS Interaction with Biomembranes
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    Chapter 28 Visualization of Endocannabinoids in the Cell
Attention for Chapter 12: Assay of NAT Activity
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Chapter title
Assay of NAT Activity
Chapter number 12
Book title
Endocannabinoid Signaling
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3539-0_12
Pubmed ID
Book ISBNs
978-1-4939-3537-6, 978-1-4939-3539-0
Authors

Toru Uyama, Natsuo Ueda

Editors

Mauro Maccarrone

Abstract

In animal tissues, N-acyltransferase (NAT) catalyzes the first reaction in the biosynthetic pathway of bioactive N-acylethanolamines, in which an acyl chain is transferred from the sn-1 position of the donor phospholipid, such as phosphatidylcholine, to the amino group of phosphatidylethanolamine, resulting in the formation of N-acylphosphatidylethanolamine. NAT has long been known to be stimulated by Ca(2+), and hence it has been referred to as Ca(2+)-dependent NAT. On the other hand, members of the phospholipase A/acyltransferase (PLA/AT) family (also known as HRAS-like suppressor family) show Ca(2+)-independent NAT activity. In this chapter, we describe (1) partial purification of Ca(2+)-dependent NAT from rat brain, (2) purification of recombinant PLA/AT-2, and (3) NAT assay using radiolabeled substrate.

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The data shown below were compiled from readership statistics for 1 Mendeley reader of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 1 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 1 100%
Readers by discipline Count As %
Medicine and Dentistry 1 100%