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Mendeley readers
Chapter title |
Assay of GTPγS Binding in Autoradiography
|
---|---|
Chapter number | 10 |
Book title |
Endocannabinoid Signaling
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3539-0_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3537-6, 978-1-4939-3539-0
|
Authors |
Marina Gabaglio, Pamela Prini, Erica Zamberletti, Tiziana Rubino, Daniela Parolaro |
Editors |
Mauro Maccarrone |
Abstract |
Autoradiography of radiolabeled GTPγS ([(35)S]GTPγS) binding is a relevant method to study the function of G protein-coupled receptors (GPCRs), in tissue sections. Here, we describe the protocol for such a binding autoradiography, suitable to investigate the functionality of CB1 receptor in tissue slices from rodent brain. |
Mendeley readers
The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 3 | 38% |
Professor > Associate Professor | 2 | 25% |
Other | 1 | 13% |
Student > Master | 1 | 13% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 25% |
Business, Management and Accounting | 1 | 13% |
Immunology and Microbiology | 1 | 13% |
Neuroscience | 1 | 13% |
Unknown | 3 | 38% |