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Endocannabinoid Signaling

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Cover of 'Endocannabinoid Signaling'

Table of Contents

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    Book Overview
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    Chapter 1 Need for Methods to Investigate Endocannabinoid Signaling
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    Chapter 2 Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
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    Chapter 3 Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS
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    Chapter 4 Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices
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    Chapter 5 Assay of CB1 Receptor Binding
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    Chapter 6 The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 7 Endocannabinoid Signaling
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    Chapter 8 A Functional Assay for GPR55: Envision Protocol
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    Chapter 9 The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 10 Assay of GTPγS Binding in Autoradiography
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    Chapter 11 Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors
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    Chapter 12 Assay of NAT Activity
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    Chapter 13 Assay of NAPE-PLD Activity
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    Chapter 14 Assay of FAAH Activity
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    Chapter 15 Assay of NAAA Activity
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    Chapter 16 Assay of DAGLα/β Activity
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    Chapter 17 Endocannabinoid Signaling
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    Chapter 18 A Sensitive and Versatile Fluorescent Activity Assay for ABHD6
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    Chapter 19 A Sensitive and Versatile Fluorescent Activity Assay for ABHD12
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    Chapter 20 Assay of Endocannabinoid Uptake
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    Chapter 21 Assay of Endocannabinoid Oxidation by Cyclooxygenase-2
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    Chapter 22 Oxygenation of Anandamide by Lipoxygenases
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    Chapter 23 Assay of Endocannabinoid Oxidation by Cytochrome P450
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    Chapter 24 Assessing Gene Expression of the Endocannabinoid System
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    Chapter 25 Western Blotting of the Endocannabinoid System
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    Chapter 26 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
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    Chapter 27 Measuring ECS Interaction with Biomembranes
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    Chapter 28 Visualization of Endocannabinoids in the Cell
Attention for Chapter 8: A Functional Assay for GPR55: Envision Protocol
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Chapter title
A Functional Assay for GPR55: Envision Protocol
Chapter number 8
Book title
Endocannabinoid Signaling
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3539-0_8
Pubmed ID
Book ISBNs
978-1-4939-3537-6, 978-1-4939-3539-0
Authors

Sharon Anavi-Goffer, Ruth A. Ross

Editors

Mauro Maccarrone

Abstract

AlphaScreen(®) SureFire(®) assay is a novel technology that combines luminescent oxygen channeling technology, nano-beads, and monocloncal antibodies to detect the level of a selected protein in a volume lower than 5 μl. This method is more sensitive compared with the traditional enzyme-linked immunosorbent assays (ELISA), and can detect an increasing number of new targets. Here, we described a method for AlphaScreen(®) SureFire(®) assay that targets ERK1/2 phosphorylation, a primary downstream signaling pathway that conveys activation of GPR55 by L-α-lysophosphatidylinositol (LPI) and certain cannabinoids.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 8 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 8 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 3 38%
Other 2 25%
Student > Master 1 13%
Unknown 2 25%
Readers by discipline Count As %
Chemical Engineering 2 25%
Biochemistry, Genetics and Molecular Biology 2 25%
Engineering 2 25%
Medicine and Dentistry 1 13%
Unknown 1 13%