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Endocannabinoid Signaling

Overview of attention for book
Cover of 'Endocannabinoid Signaling'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Need for Methods to Investigate Endocannabinoid Signaling
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    Chapter 2 Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues
  4. Altmetric Badge
    Chapter 3 Determination of 2-Arachidonoylglycerol by μSPE-LC-MS/MS
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    Chapter 4 Analysis of Omega-3 Fatty Acid Derived N-Acylethanolamines in Biological Matrices
  6. Altmetric Badge
    Chapter 5 Assay of CB1 Receptor Binding
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    Chapter 6 The Displacement Binding Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
  8. Altmetric Badge
    Chapter 7 Endocannabinoid Signaling
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    Chapter 8 A Functional Assay for GPR55: Envision Protocol
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    Chapter 9 The Cyclic AMP Assay Using Human Cannabinoid CB2 Receptor-Transfected Cells
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    Chapter 10 Assay of GTPγS Binding in Autoradiography
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    Chapter 11 Protocol to Study β-Arrestin Recruitment by CB1 and CB2 Cannabinoid Receptors
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    Chapter 12 Assay of NAT Activity
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    Chapter 13 Assay of NAPE-PLD Activity
  15. Altmetric Badge
    Chapter 14 Assay of FAAH Activity
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    Chapter 15 Assay of NAAA Activity
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    Chapter 16 Assay of DAGLα/β Activity
  18. Altmetric Badge
    Chapter 17 Endocannabinoid Signaling
  19. Altmetric Badge
    Chapter 18 A Sensitive and Versatile Fluorescent Activity Assay for ABHD6
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    Chapter 19 A Sensitive and Versatile Fluorescent Activity Assay for ABHD12
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    Chapter 20 Assay of Endocannabinoid Uptake
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    Chapter 21 Assay of Endocannabinoid Oxidation by Cyclooxygenase-2
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    Chapter 22 Oxygenation of Anandamide by Lipoxygenases
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    Chapter 23 Assay of Endocannabinoid Oxidation by Cytochrome P450
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    Chapter 24 Assessing Gene Expression of the Endocannabinoid System
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    Chapter 25 Western Blotting of the Endocannabinoid System
  27. Altmetric Badge
    Chapter 26 Quantitation of Plasma Membrane (G Protein-Coupled) Receptor Trafficking in Cultured Cells
  28. Altmetric Badge
    Chapter 27 Measuring ECS Interaction with Biomembranes
  29. Altmetric Badge
    Chapter 28 Visualization of Endocannabinoids in the Cell
Attention for Chapter 17: Endocannabinoid Signaling
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Chapter title
Endocannabinoid Signaling
Chapter number 17
Book title
Endocannabinoid Signaling
Published in
Methods in molecular biology, January 2016
DOI 10.1007/978-1-4939-3539-0_17
Pubmed ID
27245902
Book ISBNs
978-1-4939-3537-6, 978-1-4939-3539-0
Authors

Jung, Kwang-Mook, Piomelli, Daniele, Kwang-Mook Jung, Daniele Piomelli

Editors

Mauro Maccarrone

Abstract

Monoacylglycerol lipase (MGL) is a serine hydrolase involved in the biological deactivation of the endocannabinoid 2-arachidonoyl-sn-glycerol (2-AG). 2-AG is one of the main endogenous lipid agonists for cannabinoid receptors in the brain and elsewhere in the body. In the central nervous system (CNS), MGL is localized to presynaptic nerve terminals of both excitatory and inhibitory synapses, where it helps control the regulatory actions of 2-AG on synaptic transmission and plasticity. In this chapter, we describe an in vitro method to assess MGL activity by liquid chromatography/mass spectrometry (LC/MS)-based quantitation of the reaction product. This method may be used to determine the basal or altered MGL activity in various cells or animal tissues after pharmacological, genetic, or biological manipulations. In addition, this assay can be used for MGL inhibitor screening using purified recombinant enzyme or MGL-overexpressing cells.

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X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 9 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 9 100%

Demographic breakdown

Readers by professional status Count As %
Other 3 33%
Student > Doctoral Student 2 22%
Researcher 2 22%
Student > Ph. D. Student 1 11%
Student > Bachelor 1 11%
Other 0 0%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 3 33%
Biochemistry, Genetics and Molecular Biology 2 22%
Agricultural and Biological Sciences 2 22%
Medicine and Dentistry 1 11%
Chemistry 1 11%
Other 0 0%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 June 2016.
All research outputs
#15,376,252
of 22,875,477 outputs
Outputs from Methods in molecular biology
#5,350
of 13,131 outputs
Outputs of similar age
#230,972
of 393,698 outputs
Outputs of similar age from Methods in molecular biology
#544
of 1,471 outputs
Altmetric has tracked 22,875,477 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,131 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 44th percentile – i.e., 44% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,698 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 32nd percentile – i.e., 32% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,471 others from the same source and published within six weeks on either side of this one. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.

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