Chapter 1 Guidelines for the Optimization and Validation of In Situ Hybridization

Chapter 2 Overcoming Autofluorescence (AF) and Tissue Variation in Image Analysis of In Situ Hybridization

Chapter 3 Practical Application of Fluorescent In Situ Hybridization Techniques in Clinical Diagnostic Laboratories

Chapter 4 Fluorescent In Situ Hybridization Using Oligonucleotide-Based Probes

Chapter 5 Visualizing Genome Reorganization Using 3D DNA FISH

Chapter 7 Multiplexed Detection and Analysis of Low-Abundance Long Noncoding RNA Using RNAscope™ in Cultured Cells

Chapter 8 Multiplexed Quantitative In Situ Hybridization for Mammalian or Bacterial Cells in Suspension: qHCR Flow Cytometry (v3.0)

Chapter 9 Multiplexed Quantitative In Situ Hybridization for Mammalian Cells on a Slide: qHCR and dHCR Imaging (v3.0)

Chapter 10 Multiplexed Quantitative In Situ Hybridization with Subcellular or Single-Molecule Resolution Within Whole-Mount Vertebrate Embryos: qHCR and dHCR Imaging (v3.0)

Chapter 11 Hybridization Chain Reaction for Quantitative and Multiplex Imaging of Gene Expression in Amphioxus Embryos and Adult Tissues

Chapter 12 RNAscope™ Multiplex Detection in Zebrafish

Chapter 13 Duplex In Situ Hybridization of Virus Nucleic Acids in Plant Tissues Using RNAscope ®

Chapter 14 Automated ISH for Validated Histological Mapping of Lowly Expressed Genes

Chapter 15 Automation of Multiplexed RNAscope Single-Molecule Fluorescent In Situ Hybridization and Immunohistochemistry for Spatial Tissue Mapping

Chapter 16 Automated Co-in Situ Hybridization and Immunofluorescence Using Archival Tumor Tissue

Chapter 17 Automated Five-Color Multiplex Co-detection of MicroRNA and Protein Expression in Fixed Tissue Specimens

Chapter 18 Mixed Multiplex Staining: Automated RNAscope™ and OPAL™ for Multiple Targets

Chapter 19 Simultaneous Visualization of RNA and Protein Expression in Tissue Using a Combined RNAscope™ In Situ Hybridization and Immunofluorescence Protocol

Chapter 20 In Situ Sequencing: A High-Throughput, Multi-Targeted Gene Expression Profiling Technique for Cell Typing in Tissue Sections

Chapter 21 GeoMx™ RNA Assay: High Multiplex, Digital, Spatial Analysis of RNA in FFPE Tissue

Chapter 22 In Situ Point Mutation Detection in FFPE Colorectal Cancers Using the BaseScope Assay

Chapter 23 Using In Situ Padlock Probe Technology to Detect mRNA Splice Variants in Tumor Cells

Chapter 24 Automated One-Double-Z Pair BaseScope™ for CircRNA In Situ Hybridization